Human IL-17F Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-17F in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-17A, recombinant mouse IL-17F, or recombinant rat IL-17F is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 775620
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-17F
    Gly21-Thr153
    Accession # Q96PD4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑17F-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 0.1-0.6 μg/mL in the presence of 25 ng/mL Recombinant Human IL‑17F.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL-6 Secretion Induced by IL‑17F and Neutralization by Human IL‑17F Antibody. Recombinant Human IL‑17F induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent response (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). Under these conditions, IL-6 secretion elicited by IL‑17F is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13352). The ND50 is typically 0.1-0.6 μg/mL.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine‑knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Human IL-17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16 - 30%) with IL-17B, C, D and E. Human and mouse IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T-cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds IL-17B and IL-17E. IL-17 RC binds IL-17A and IL-17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL-17F are similar to those of IL‑17. IL‑17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL‑17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

  • References:
    1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
    2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
    3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
    4. Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
    5. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  • Long Name:
    Interleukin 17F
  • Entrez Gene IDs:
    112744 (Human); 257630 (Mouse); 301291 (Rat)
  • Alternate Names:
    Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24
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Average Rating: 5 (Based on 1 review)

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Summary

ApplicationELISA
Sample TestedCHO Chinese hamster ovary cell line
SpeciesMouse

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