Human IL-28B/IFN-lambda 3 Antibody

Catalog # Availability Size / Price Qty
MAB52591-100
MAB52591-SP

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Human IL-28B/IFN-lambda 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑28B/IFN‑ lambda 3 in ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 567107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL‑28B/IFN‑ lambda 3
Arg30-Val200 (Lys74Arg)
Accession # AAN28264
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑28B/IFN‑ lambda 3 Monoclonal Antibody (Catalog # MAB52593).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28B/IFN-lambda 3

IL-28B (also named interferon-lambda 3, IFN-lambda 3), IL-28A (IFN-lambda 2) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Human IL-28B cDNA encodes a 200 amino acid (aa) protein with a 25 aa signal peptide and a 175 aa mature protein that lacks N-glycosylation sites. Mature human IL-28B shares 64% and 75% aa sequence identity with mouse and canine IL-28B, respectively, and is active across species (5). Human IL-28B shares 94% and 69% aa identity with human IL-28A and IL-29, respectively (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce anti-viral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust anti-viral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
  5. Ank, N. et al. (2006) J. Virol. 80:4501.
  6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
  7. Siebler, J. et al. (2007) Gastroenterology 132:358.
  8. Meager, A. et al. (2005) Cytokine 31:109.
  9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  10. Sato, A. et al. (2006) J. Immunol. 176:7686.
  11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
Long Name
Interleukin 28B
Entrez Gene IDs
282617 (Human); 338374 (Mouse)
Alternate Names
interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3

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