IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.
Human IL-3 Antibody (4815R)
R&D Systems | Catalog # MAB603R
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, Bioassay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # 4815R
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant human IL-3
Ala20-Phe152
Accession # P08700
Ala20-Phe152
Accession # P08700
Specificity
Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, recombinant mouse (rm) IL-1 beta, rhIL-2, rhIL-3 R alpha, rhIL-3 R beta, rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF‑ beta 1, porcineTGF‑ beta 1, rhTGF‑ beta 1, porcine TGF‑ beta 2, porcine TGF‑ beta 1.2, rhTNF-alpha, or rhTNF-beta was observed. Does not neutralize the biological activity ofrmIL‑3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-3 Antibody (4815R)
Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 Antibody.
Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Recombinant Monoclonal Antibody (Catalog # MAB603R). The ND50 is typically 0.1-0.3 µg/mL.Applications for Human IL-3 Antibody (4815R)
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3.
Human IL-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-3
Long Name
Interleukin 3
Alternate Names
IL3, MCGF, MULTI-CSF
Gene Symbol
IL3
UniProt
Additional IL-3 Products
Product Documents for Human IL-3 Antibody (4815R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-3 Antibody (4815R)
For research use only
Citations for Human IL-3 Antibody (4815R)
Powered by Bioz
Customer Reviews for Human IL-3 Antibody (4815R)
There are currently no reviews for this product. Be the first to review Human IL-3 Antibody (4815R) and earn rewards!
Have you used Human IL-3 Antibody (4815R)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
