Human IL-31 DuoSet ELISA

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Human IL-31 ELISA Standard Curve
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Product Details
Citations (8)
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Human IL-31 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-31. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.


Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human IL-31 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-31

Interleukin 31 (IL-31) is a secreted, T-cell-derived, 24-kDa, short-chain member of the alpha-helical family of cytokines. The mature region is 141 amino acids (aa) in length and exhibits four alpha-helices. Human and mouse IL-31 are highly divergent at 24% aa sequence identity. The receptor for IL-31 is found on keratinocytes and monocytes. It is a heterodimer composed of the Oncostatin M receptor and a novel gp130-related molecule. IL-31 may be involved in pruritis, or skin itching.

Long Name:
Interleukin 31
Entrez Gene IDs:
386653 (Human); 76399 (Mouse)
Alternate Names:
IL31; IL-31; IL-31interleukin-31; interleukin 31

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.


Citations for Human IL-31 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Rare Phytocannabinoids Exert Anti-Inflammatory Effects on Human Keratinocytes via the Endocannabinoid System and MAPK Signaling Pathway
    Authors: D Tortolani, C Di Meo, S Standoli, F Ciaramella, S Kadhim, E Hsu, C Rapino, M Maccarrone
    International Journal of Molecular Sciences, 2023-02-01;24(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Effects of Dupilumab on Itch-Related Events in Atopic Dermatitis: Implications for Assessing Treatment Efficacy in Clinical Practice
    Authors: R Kishi, S Toyama, M Tominaga, Y Kamata, E Komiya, T Kaneko, Y Suga, K Takamori
    Cells, 2023-01-05;12(2):.
    Species: Human
    Sample Types: Serum
  3. miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer
    Authors: D Parashar, A Geethadevi, MR Aure, J Mishra, J George, C Chen, MK Mishra, A Tahiri, W Zhao, B Nair, Y Lu, LS Mangala, C Rodriguez-, G Lopez-Bere, AKS Camara, M Liang, JS Rader, R Ramchandra, M You, AK Sood, VN Kristensen, GB Mills, S Pradeep, P Chaluvally
    Cell Rep, 2019-12-24;29(13):4389-4406.e10.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Effect of Resveratrol-Enriched Rice on Skin Inflammation and Pruritus in the NC/Nga Mouse Model of Atopic Dermatitis
    Authors: MC Kang, K Cho, JH Lee, L Subedi, S Yumnam, SY Kim
    Int J Mol Sci, 2019-03-21;20(6):.
    Species: Mouse
    Sample Types: Serum
  5. The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction
    Authors: K Yamamura, T Uruno, A Shiraishi, Y Tanaka, M Ushijima, T Nakahara, M Watanabe, M Kido-Nakah, I Tsuge, M Furue, Y Fukui
    Nat Commun, 2017-01-09;8(0):13946.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. IL-31 associated with coronary artery lesion formation in Kawasaki disease.
    Authors: Tseng W, Lo M, Guo M, Hsieh K, Chang W, Kuo H
    PLoS ONE, 2014-08-14;9(8):e105195.
    Species: Human
    Sample Types: Plasma
  7. IL-31 Serum Protein and Tissue mRNA Levels in Patients with Atopic Dermatitis.
    Authors: Kim S, Kim HJ, Yang HS
    Ann Dermatol, 2011-11-03;23(4):468-73.
    Species: Human
    Sample Types: Serum
  8. Correlation of IL-31 serum levels with severity of atopic dermatitis.
    Authors: Raap U, Wichmann K, Bruder M, Stander S, Wedi B, Kapp A, Werfel T
    J. Allergy Clin. Immunol., 2008-08-01;122(2):421-3.
    Species: Human
    Sample Types: Serum


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