Human IL-9R Antibody

R&D Systems | Catalog # AF290

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-9R Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9 R
Val40-Pro270
Accession # Q01113

Specificity

Detects human IL-9 R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) IL-2 sR gamma, rhIL-5 sR alpha, and rhIL-6 sR is observed and 10% cross-reactivity with rhIL‑2 sR beta is observed and 5% cross‑reactivity with rhIL‑1 sRII, rhIL-4 sR, and rhIL-10 sR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-9R Antibody

Detection of IL-9R by Western Blot

Detection of IL-9R by Western Blot

Ucma/GRP−/− mVSMCs show increased expression of osteo/chondrogenic markers. Cells were treated with control (CM) or osteogenic medium (OM, with 2.6 mM phosphate) for 12 days. Panels (A–D) qPCR analysis of osteo/chondrogenic gene expression, n = 4–7. Statistical significance was assessed using t-test (Osteocalcin) or ANOVA (E) Western blotting analysis of osteo/chondrogenic markers. Both WT and KO cells were treated with OM. Figure shows full western blots obtained by cutting of a full-sized membrane prior to antibody incubations. Numbers in the middle show molecular weights of the protein standard. Numbers on the right show the expected molecular weights of the proteins. Panels F–I quantification of western blotting results from panel E, n = 3. Statistical significance was assessed using t-test. All graphs show mean + SD. *p between 0.05–0.01, **p between 0.001–0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29563538), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-9R Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑9 R Subunit (Catalog # 290-RNS)

Reviewed Applications

Read 1 review rated 3 using AF290 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-9R

The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha ) type I transmembrane subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. Human and mouse IL-9 receptors share 53% amino acid sequence identity, and contain 468 and 533 amino acids, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.

Long Name

Interleukin 9 Receptor

Alternate Names

CD129, IL-9 R, IL9R

Entrez Gene IDs

3581 (Human); 16199 (Mouse)

Gene Symbol

IL9R

UniProt

Q01113

Additional IL-9R Products

Product Documents for Human IL-9R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-9R Antibody

For research use only

Citations for Human IL-9R Antibody

Customer Reviews for Human IL-9R Antibody (1)

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  • Human IL-9R Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with mAb (2901) and protein (290-RNS) to detect IL9R in human serum and plasma. This combination could not detect any IL9R in our samples but generated a good standard curve.
    Human IL-9R Antibody AF290

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