Human IL-9R Antibody

Catalog # Availability Size / Price Qty
AF290
AF290-SP
Product Details
FAQs
Supplemental Products
Reviews (1)

Human IL-9R Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-9 R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human (rh) IL-2 sR gamma, rhIL-5 sR alpha, and rhIL-6 sR is observed and 10% cross-reactivity with rhIL‑2 sR beta is observed and 5% cross‑reactivity with rhIL‑1 sRII, rhIL-4 sR, and rhIL-10 sR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9 R
Val40-Pro270
Accession # Q01113
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑9 R Subunit (Catalog # 290-RNS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9R

The specific high affinity functional IL-9 receptor complex consists of a ligand-specific IL-9 R( alpha ) type I transmembrane subunit and the common gamma chain that is shared with the receptors for IL-2, IL-4, IL-7 and IL-15. Both the IL-9 R subunit and the common gamma chain are members of the hematopoietin receptor superfamily. Human and mouse IL-9 receptors share 53% amino acid sequence identity, and contain 468 and 533 amino acids, respectively. As a result of alternative splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.

Long Name
Interleukin 9 Receptor
Entrez Gene IDs
3581 (Human); 16199 (Mouse)
Alternate Names
CD129 antigen; CD129; IL-9 R; IL-9 receptor; IL9R; IL-9R; interleukin 9 receptor; interleukin-9 receptor

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human IL-9R Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb (2901) and protein (290-RNS) to detect IL9R in human serum and plasma. This combination could not detect any IL9R in our samples but generated a good standard curve.