Human Insulysin/IDE Antibody
Human Insulysin/IDE Antibody Summary
Accession # P14735
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-alpha in vitro (1). In addition, IDE has been shown to degrade the amyloid beta (A beta ) peptide, which polymerizes into the plaques associated with Alzheimer's disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer's disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in A beta degradation in the brain, resulting in increased cerebral accumulation of A beta peptide. This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain.
- Affholter, J. A. et al. (1988) Science 242:1415.
- Duckworth, W.C. et al. (1998) Endocr. Rev. 19:608.
- Akiyama, H. et al. (1990) Biochem. Biophys. Res. Commun. 170:1325.
- Selkoe, D.J. (2001) Neuron 32:177.
- Ghosh, S. et al. (2000) Am. J. Hum. Genet. 67:1174.
- Farris, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4162.
Citations for Human Insulysin/IDE Antibody
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Citations: Showing 1 - 2
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Insight into PreImplantation Factor (PIF*) mechanism for embryo protection and development: target oxidative stress and protein misfolding (PDI and HSP) through essential RIKP [corrected] binding site.
Authors: Barnea E, Lubman D, Liu Y, Absalon-Medina V, Hayrabedyan S, Todorova K, Gilbert R, Guingab J, Barder T
PLoS ONE, 2014-07-01;9(7):e100263.
Sample Types: Embryo
Applications: Array Development
Down-regulation of insulin-degrading enzyme by presenilin 1 V97L mutant potentially underlies increased levels of amyloid beta 42.
Authors: Qin W, Jia J
Eur. J. Neurosci., 2008-05-01;27(9):2425-32.
Sample Types: Cell Lysates
Applications: Western Blot
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