Human Integrin alpha 5/CD49e Antibody

Name: Human Integrin alpha 5/CD49e Antibody
Brand: R&D Systems
SKU: MAB18642
Price: 429 USD
Availability: InStock

R&D Systems | Catalog # MAB18642

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 612557

View all Integrin alpha 5/CD49e Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648

Specificity

Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Integrin alpha 5, recombinant human (rh) Integrin alpha 1, 2, 3, 4, 6, 7x2, 8, 9, 10, 11, M, X, D, or rhIntegrin beta 1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Integrin alpha 5/CD49e Antibody

Detection of Human Integrin alpha 5/CD49e by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a5/CD49e Monoclonal Antibody (Catalog # MAB18642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a5/CD49e at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Integrin alpha 5/CD49e Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 5/CD49e

Integrin alpha 5, also known as CD49e and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of Integrin alpha 5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a beta -propeller domain. Furin cleavage of the Integrin alpha 5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin alpha 5 chain. Integrin alpha 5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha 5 shares 92% aa sequence identity with mouse Integrin alpha 5 ECD.