Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Integrin alpha 5, recombinant human (rh) Integrin alpha 1, 2, 3, 4, 6, 7x2, 8, 9, 10, 11, M, X, D, or rhIntegrin beta 1 is observed.
Monoclonal Mouse IgG2A Clone # 612557
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 5/CD49e Phe42-Tyr995 Accession # P08648
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Integrin alpha 5/CD49e by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin alpha 5/CD49e Monoclonal Antibody (Catalog # MAB18642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 5/CD49e at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 5/CD49e
Integrin alpha 5, also known as CD49e and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of Integrin alpha 5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a beta -propeller domain. Furin cleavage of the Integrin alpha 5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin alpha 5 chain. Integrin alpha 5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha 5 shares 92% aa sequence identity with mouse Integrin alpha 5 ECD.
Entrez Gene IDs:
3678 (Human); 16402 (Mouse)
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5
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