Human Integrin  alpha 5/CD49e Antibody

Catalog # Availability Size / Price Qty
MAB18642
MAB18642-SP
Detection of Human Integrin  alpha 5/CD49e by Western Blot.
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Human Integrin  alpha 5/CD49e Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Integrin alpha 5, recombinant human (rh) Integrin alpha 1, 2, 3, 4, 6, 7x2, 8, 9, 10, 11, M, X, D, or rhIntegrin beta 1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 612557
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin a5/CD49e antibody by Western Blot. View Larger

Detection of Human Integrin alpha 5/CD49e by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a5/CD49e Monoclonal Antibody (Catalog # MAB18642) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a5/CD49e at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha 5, also known as CD49e and vitronectin receptor subunit  alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of Integrin alpha 5, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) consensus repeats that fold into a beta -propeller domain. Furin cleavage of the Integrin alpha 5 ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit Integrin alpha 5 chain. Integrin alpha 5-containing integrins bind multiple ECM molecules, including Vitronectin, Osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha 5 shares 92% aa sequence identity with mouse Integrin alpha 5 ECD.

Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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