Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells.
Integrin alpha V/CD51 was detected in immersion fixed rat mesenchymal stem cells using Mouse Anti-Human Integrin alpha V/ CD51 Monoclonal Antibody (Catalog # MAB12191) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha V/CD51
Integrin alpha V (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human alpha V is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta -propellor domain (aa 46‑483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V shares 92% and 90% aa sequence identity with mouse and rat alpha V ECD, respectively.
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