Human Integrin  beta 2/CD18 Antibody

Catalog # Availability Size / Price Qty
AF1730
AF1730-SP
Human Integrin  beta 2/CD18 Antibody in Immunocytochemistry
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Product Details
Citations (9)
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Human Integrin  beta 2/CD18 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, recombinant mouse Integrin beta 2, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 5, rhIntegrin beta 6, and rhIntegrin beta 7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2
Gln23-Asn700
Accession # AAA59490
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Integrin  beta 2/CD18 in Human PBMCs. View Larger

Integrin  beta 2/CD18 in Human PBMCs. Integrin  beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin  beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Entrez Gene IDs
3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)
Alternate Names
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

Product Datasheets

Citations for Human Integrin  beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. The Extracellular Domain of the β2 Integrin β Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity
    Authors: LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch
    MBio, 2019;10(4):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis
    Authors: MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht
    Sci Adv, 2019;5(3):eaau7518.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Biomimetic post-capillary venule expansions for leukocyte adhesion studies
    Authors: BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff
    Sci Rep, 2018;8(1):9328.
  4. Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
    Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Bystander cells enhance NK cytotoxic efficiency by reducing search time
    Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
    Sci Rep, 2017;7(0):44357.
    Species: Human
    Sample Types: Whole Cells
    Applications: Migration Assay
  6. An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism.
    Authors: Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L, Jansen T, van de Veerdonk F, Williams D, Joosten L, Dinarello C, Netea M
    Cytokine, 2015;71(2):215-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014;12(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
    Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
    J. Biol. Chem., 2011;286(9):7070-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
    Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
    Dig. Dis. Sci., 2007;52(10):2775-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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