Human Integrin beta 2/CD18 Antibody

AF1730 9 Citations Datasheet / COA / SDS

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AF1730
AF1730-SP

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Human Integrin beta 2/CD18 Antibody in Immunocytochemistry

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Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin beta 2/CD18 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, recombinant mouse Integrin beta 2, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 5, rhIntegrin beta 6, and rhIntegrin beta 7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin beta 2
Gln23-Asn700
Accession # AAA59490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells

Adhesion Blockade

The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 10⁴ cells/well) to immobilized Recombinant Human ICAM-1/CD54 Fc Chimera (Catalog # 720-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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Integrin beta 2/CD18 in Human PBMCs. Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1730) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Entrez Gene IDs

3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)

Alternate Names

959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Integrin beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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The Extracellular Domain of the Î²2 Integrin Î² Subunit (CD18) Is Sufficient for Escherichia coli Hemolysin and Aggregatibacter actinomycetemcomitans Leukotoxin Cytotoxic Activity
Authors: LC Ristow, V Tran, KJ Schwartz, L Pankratz, A Mehle, JD Sauer, RA Welch
MBio, 2019;10(4):.
Species: Human
Sample Types: Protein
Applications: Western Blot
Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis
Authors: MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht
Sci Adv, 2019;5(3):eaau7518.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Biomimetic post-capillary venule expansions for leukocyte adhesion studies
Authors: BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff
Sci Rep, 2018;8(1):9328.
Synthesis of Human Neutrophil Extracellular Traps Contributes to Angiopoietin-Mediated In Vitro Proinflammatory and Proangiogenic Activities
Authors: SS Lavoie, E Dumas, B Vulesevic, PE Neagoe, M White, MG Sirois
J. Immunol., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Bystander cells enhance NK cytotoxic efficiency by reducing search time
Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
Sci Rep, 2017;7(0):44357.
Species: Human
Sample Types: Whole Cells
Applications: Migration Assay
An anti-inflammatory property of Candida albicans beta-glucan: Induction of high levels of interleukin-1 receptor antagonist via a Dectin-1/CR3 independent mechanism.
Authors: Smeekens S, Gresnigt M, Becker K, Cheng S, Netea S, Jacobs L, Jansen T, van de Veerdonk F, Williams D, Joosten L, Dinarello C, Netea M
Cytokine, 2015;71(2):215-22.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
Cell Commun Signal, 2014;12(0):36.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Complement Receptor Mac-1 Is an Adaptor for NB1 (CD177)-mediated PR3-ANCA Neutrophil Activation.
Authors: Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
J. Biol. Chem., 2011;286(9):7070-81.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC