Human IRAK1 Antibody

Catalog # Availability Size / Price Qty
AF4048
AF4048-SP
Detection of Human IRAK1 by Western Blot.
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Product Details
Citations (1)
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Human IRAK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRAK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK2 and rhIRAK4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IRAK1
Met1-Ser693
Accession # AAH54000
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IRAK1 antibody by Western Blot. View Larger

Detection of Human IRAK1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (10 ng/lane) were included. A specific band for IRAK1 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRAK1

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4.

Long Name
Interleukin-1 Receptor-Associated Kinase 1
Entrez Gene IDs
3654 (Human); 16179 (Mouse); 363520 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; Il1rak; interleukin-1 receptor-associated kinase 1; IRAK1; IRAK-1; IRAKPelle homolog; Pelle; Plpk

Product Datasheets

Citation for Human IRAK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020;0(6):679-686.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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