Human IRAK1 Antibody Summary
Accession # AAH54000
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IRAK1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (10 ng/lane) were included. A specific band for IRAK1 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4.
Citation for Human IRAK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
Chin. Med. J., 2020;0(6):679-686.
Sample Types: Cell Lysate
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human IRAK1 Antibody
There are currently no reviews for this product. Be the first to review Human IRAK1 Antibody and earn rewards!
Have you used Human IRAK1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image