Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IRAK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRAK1
Met1-Ser693
Accession # AAH54000

Specificity

Detects human IRAK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK2 and rhIRAK4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IRAK1 Antibody

Detection of Human IRAK1 antibody by Western Blot.

Detection of Human IRAK1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (10 ng/lane) were included. A specific band for IRAK1 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IRAK1 by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK1 at approximately 83 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IRAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4048). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IRAK1 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HT-29 human colon adenocarcinoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRAK1

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that regulate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. All IRAKs are multidomain proteins, containing a conserved N-terminal death domain as well as a central kinase domain that is only active in IRAK1 and IRAK4.

Long Name

Interleukin-1 Receptor-Associated Kinase 1

Alternate Names

Il1rak, Pelle, Plpk

Entrez Gene IDs

3654 (Human); 16179 (Mouse); 363520 (Rat)

Gene Symbol

IRAK1

UniProt

AAH54000

Additional IRAK1 Products

Product Documents for Human IRAK1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRAK1 Antibody

For research use only

Citations for Human IRAK1 Antibody

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