Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 996308
View all iRHOM2/RHBDF2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human iRHOM2/RHBDF2
Accession # Q6PJF5

Specificity

Detects huma iRHOM2/RHBDF2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human iRHOM2/RHBDF2 Antibody

Detection of Human iRHOM2/RHBDF2 antibody by Western Blot.

Detection of Human iRHOM2/RHBDF2 by Western Blot.

Western blot shows lysates of LoVo human colorectal adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human iRHOM2/RHBDF2 Monoclonal Antibody (Catalog # MAB10048) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iRHOM2/RHBDF2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human iRHOM2/RHBDF2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: LoVo human colorectal adenocarcinoma cell line and MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: iRHOM2/RHBDF2

Human Inactive rhomboid protein 2 (RHBDF2 or IRHOM2) is a highly conserved seven-transmembrane Rhomboid protease-like protein which is inactive, it has no protease activity, but has been implicated in the secretion of several ligands of the epidermal growth factor receptor (EGFR), probably by regulating trafficking and stability of key protein membrane like ADAM17 and STING. The RHBDF2 protein plays an important role in the secretion of tumor necrosis factor alpha and has been implicated in familial esophageal cancer. Gain-of-function (GOF) mutations in the RHBDF2 gene constitutively activate EGFR signaling to cause tylosis with esophageal cancer syndrome.

Long Name

Rhomboid 5 Homolog 2

Alternate Names

iRHOM2, RHBDL5, RHBDL6, TOCG

Entrez Gene IDs

79651 (Human); 217344 (Mouse); 303690 (Rat)

Gene Symbol

RHBDF2

UniProt

Q6PJF5 (Mouse)

Additional iRHOM2/RHBDF2 Products

Product Documents for Human iRHOM2/RHBDF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human iRHOM2/RHBDF2 Antibody

For research use only

Citations for Human iRHOM2/RHBDF2 Antibody

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