Human Irisin/FNDC5 Antibody

Catalog # Availability Size / Price Qty
MAB94202-100
MAB94202-SP
Detection of Irisin/FNDC5 in Human Heart.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Irisin/FNDC5 Antibody Summary

Specificity
Detects Irisin peptide in direct ELISA.
Source
Monoclonal Mouse IgG2A Clone # 1056126
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide covering the C-term of the Irisin peptide sequences of the FNDC5 gene.
Glu97-Glu143
Accession # Q8NAU1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Heart.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Irisin/FNDC5 in Human Heart. Irisin/FNDC5 was detected in immersion fixed paraffin-embedded sections of Human Heart using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94202) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated disks. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

References
  1. Bostrom, P. et al. (2012) Nature 481:463.
  2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
  3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
  4. Teufel, A. et al. (2002) Gene 297:79.
  5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
  6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
  7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
  8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
  9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
  10. Dun, S.L. et al. (2013) Neuroscience 240:155.
  11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
  12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
  13. Moon, H.S. et al. (2013) Metabolism 62:1131.
  14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.
Long Name
Fibronectin Type III Domain Containing 5
Entrez Gene IDs
252995 (Human); 384061 (Mouse); 260327 (Rat)
Alternate Names
fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Irisin/FNDC5 Antibody

There are currently no reviews for this product. Be the first to review Human Irisin/FNDC5 Antibody and earn rewards!

Have you used Human Irisin/FNDC5 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review