Human JAM-B/VE-JAM Antibody Summary
Accession # P57087
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of JAM-B/VE-JAM in HEK293 Human Cell Line Transfected with Human JAM-B/VE-JAM and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human JAM-B/VE-JAM or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human JAM-B/VE-JAM Monoclonal Antibody (Catalog # MAB10743) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (3, 5). Human JAM-B cDNA predicts a 298 amino acid (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site. Human JAM-B shares approximately 79% aa sequence homology with its mouse homologue. It also shares approximately 35% aa sequence homology with human JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the Integrin alpha 4 beta 1. However, the JAM-B/ alpha 4 beta 1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).
The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred in the literature variously as JAM-B or JAM-3. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.
- Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
- Aurand-Lions, M. et al. (2001) Blood 98:3699.
- Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
- Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
- Liang, T. et al. (2002) J. Immunol. 168:1618.
- Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
- Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.
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