Human Jarid1A/KDM5A Antibody

Catalog #: MAB11705 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11705-100
MAB11705-SP

Detection of Human Jarid1A by Western Blot.

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All Jarid1A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Jarid1A/KDM5A Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human Jarid1A/KDM5a protein in Direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1102542

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Jarid1A/KDM5A
Lys127-Ser287
Accession # P29375

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

NTera-2 human testicular embryonic carcinoma cell line

Simple Western

2-10 µg/mL

NTera-2 human testicular embryonic carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Jarid1A by Western Blot. Western Blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Jarid1A at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human Jarid1A by Simple Western^TM. Left: Simple Western lane view shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.75 mg/ml. A specific band was detected for Jarid1A at approximately 191 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 66-440kDa separation system. Right: Simple Western electropherogram showing the same Mouse Anti-Human Jarid1A Monoclonal Antibody (Catalog # MAB11705) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in the NTera‑2 human testicular embryonic carcinoma cell line.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jarid1A

Jarid1A (also known as KDM5A) is a protein of approximately 175kDa that belongs to the Jumonji/ARID1 (JARID) protein family. It functions primarily as a lysine demethylase, involved in the regulation of histone H3 lysine 4 (H3K4) demethylation, thereby playing a crucial role in chromatin remodeling and epigenetic regulation. Jarid1A is implicated in multiple biological processes, including cell cycle progression, differentiation, and development. Dysregulation of Jarid1A is associated with various pathologies, particularly its overexpression in several types of cancer, where it contributes to tumorigenesis, cancer cell proliferation, and metastasis. It is also linked to altered gene expression profiles in neurological disorders. The role of Jarid1A in transcriptional regulation highlights its potential as a therapeutic target and biomarker for cancer and other diseases involving epigenetic dysregulation.

References

Harmeyer KM, Facompre ND, Herlyn M, Basu D. JARID1 Histone Demethylases: Emerging Targets in Cancer. Trends Cancer. 2017 Oct;3(10):713-725. doi: 10.1016/j.trecan.2017.08.004. Epub 2017 Sep 12. PMID: 28958389; PMCID: PMC5679451.
Yang GJ, Zhu MH, Lu XJ, Liu YJ, Lu JF, Leung CH, Ma DL, Chen J. The emerging role of KDM5A in human cancer. J Hematol Oncol. 2021 Feb 17;14(1):30. doi: 10.1186/s13045-021-01041-1. PMID: 33596982; PMCID: PMC7888121.

Long Name

Lysine-specific demethylase 5A

Entrez Gene IDs

5927 (Human); 214899 (Mouse)

Alternate Names

EC 1.14.11; EC 1.14.11.-; EC 1.14.11.67; Histone demethylase JARID1A; Jarid1A; JARID1AJumonji, AT rich interactive domain 1A (RBP2-like); Jumonji, AT rich interactive domain 1A (RBBP2-like); Jumonji/ARID domain-containing protein 1A; KDM5A; lysine (K)-specific demethylase 5A; RBBP2; RBBP-2; RBBP2lysine-specific demethylase 5A; RBP2; RBP2retinoblastoma binding protein 2; Retinoblastoma-binding protein 2jumonji, AT rich interactive domain 1A