Human JunB Antibody

Catalog # Availability Size / Price Qty
Detection of Human JunB by Western Blot.
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Product Details
Supplemental Products

Human JunB Antibody Summary

Species Reactivity
Detects human JunB in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) JunD or rhc-Jun is observed.
Monoclonal Mouse IgG1 Clone # 512313
Protein A or G purified from hybridoma culture supernatant
E. coli-drived recombinant human JunB
Asp145-Val246 (predicted)
Accession # P17275
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human JunB antibody by Western Blot. View Larger

Detection of Human JunB by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human JunB Monoclonal Antibody (Catalog # MAB4456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for JunB at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunB

JunB is a member of the bzip Jun family of transcription factors. JunB binds as a homo or heterodimer, with other members of the Jun/Fos family, to the DNA element TGA[CG]TCA. Knock out mice indicate that JunB is involved in erythroid differentiation and hematopoietic stem cell viability.

Long Name
JunB Proto-oncogene
Entrez Gene IDs
3726 (Human); 16477 (Mouse); 24517 (Rat)
Alternate Names
activator protein 1; AP-1; jun B proto-oncogene; JunB; transcription factor jun-B

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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