Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 364932
View all Kallikrein 12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
Ala18-Asn248
Accession # Q9UKR0

Specificity

Detects human Kallikrein 12 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh)  Kallikrein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, rhKallikrein  beta 1, rhHGF Activator, rhFactor VII, rhFactor X, rhFactor XI, rhThrombin or rhuPA-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Kallikrein 12 Antibody

Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody.

Neutralization of Kallikrein 12 Activity by Human Kallikrein 12 Antibody.

The cleavage of Boc-VPR-AMC (0.1 mM, Catalog # ES011) by Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) is measured after preincubation with increasing concentrations of Human Kallikrein 12 Monoclonal Antibody (Catalog # MAB40341). The ND50 is typically 0.1 µg/mL.

Applications for Human Kallikrein 12 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 12 (Catalog # 3095-SE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Kallikrein 12 (Catalog # 3095-SE) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 12 (0.1 µg/mL, Catalog # 3095-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (0.1 mM, Catalog # ES011). The Neutralization Dose (ND50) is typically 0.1 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 12

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family (1). It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat.

References

  1. Yousef, G.M. et al. (2000) Genomics. 69:331.
  2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Alternate Names

KLK-L5, KLK12

Entrez Gene IDs

43849 (Human)

Gene Symbol

KLK12

UniProt

Q9UKR0

Additional Kallikrein 12 Products

Product Documents for Human Kallikrein 12 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 12 Antibody

For research use only

Citations for Human Kallikrein 12 Antibody

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