Human KGF/FGF-7 Antibody

Catalog # Availability Size / Price Qty
MAB251-SP
MAB251-500
MAB251-100
Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human KGF/FGF-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KGF/FGF‑7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF-3, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF acidic, or rhFGF basic is observed.
Source
Monoclonal Mouse IgG1 Clone # 29522
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human KGF/FGF‑7
Cys32-Thr194
Accession # P21781
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Human KGF/FGF-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human KGF/FGF‑7 Biotinylated Antibody (Catalog # BAF251)

Standard: Recombinant Human KGF/FGF-7 Protein (Catalog # 251-KG)

Neutralization
Measured by its ability to neutralize KGF/FGF‑7-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 100 ng/mL Recombinant Human KGF/FGF‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody. View Larger

Cell Proliferation Induced by KGF/FGF‑7 and Neutralization by Human KGF/FGF‑7 Antibody. Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human KGF/FGF-7 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human KGF/FGF-7 Monoclonal Antibody (Catalog # MAB251). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1‑4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine, and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

References
  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.
Long Name
Keratinocyte Growth Factor
Entrez Gene IDs
2252 (Human); 403915 (Canine)
Alternate Names
FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)

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Citations for Human KGF/FGF-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005-02-16;132(6):1223-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Induction of keratinocyte growth factor 1 Expression by lipopolysaccharide is regulated by CD-14 and toll-like receptors 2 and 4.
    Authors: Putnins EE, Sanaie AR, Wu Q
    Infect. Immun., 2002-12-01;70(12):6541-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Human KGF/FGF-7 Antibody
By Anonymous on 12/06/2017
Application: ELISA Sample Tested: Recombinant protein Species: Human