Human KIR3DL1 Antibody

  (3 citations)     
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Product Details
Citations (3)
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  • Species Reactivity
    Human
  • Specificity
    Detects human KIR3DL1 on transfected BaF/3 cells. When tested against a panel of transfectants, no cross-reactivity with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL2, or 3DS1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 177407
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human KIR3DL1
    Accession # P43629
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood CD56+ natural killer cells
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: KIR3DL1

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1‑3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40‑48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

  • References:
    1. Colonna, M. and J. Samaridis (1995) Science 268:405.
    2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
    3. Uhrberg, M. et al. (1997) Immunity 7:753.
    4. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
    5. Thananchai, H. et al. (2007) J. Immunol. 178:33.
    6. Martin, M.P. et al. (2007) Nat. Genet. 39:733.
    7. Pando, M.J. et al. (2003) J. Immunol. 171:6640.
    8. Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
    9. Wilson, E.B. et al. (2007) Immunogenetics 59:641.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 1
  • Entrez Gene IDs:
    3811 (Human)
  • Alternate Names:
    AMB11; CD158 antigen-like family member E; CD158e antigen; CD158E; CD158e1; CD158e1/2; CD158e2; cl-11; cl-2; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3; killer Ig receptor; KIR antigen 3DL1; KIR3DL1; KIR3DL1/S1; KIR3DS1; MGC119726; MGC119728; MGC126589; MGC126591; MHC class I NK cell receptor; Natural killer-associated transcript 3; Nkat3; NKAT-3; NKAT3KIR; NKB1; NKB1B; NKB1CD158E1; NK-receptor; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Clinical impact of NK-cell reconstitution after reduced intensity conditioned unrelated cord blood transplantation in patients with acute myeloid leukemia: analysis of a prospective phase II multicenter trial on behalf of the Soci�t� Fran�aise de Greffe de Moelle Osseuse et Th�rapie Cellulaire and Eurocord
    Authors: S Nguyen, A Achour, L Souchet, S Vigouroux, P Chevallier, S Furst, A Sirvent, JO Bay, G Socié, P Ceballos, A Huynh, J Cornillon, S Francois, F Legrand, I Yakoub-Agh, G Michel, N Maillard, G Margueritt, S Maury, M Uzunov, CE Bulabois, M Michallet, L Clement, C Dauriac, K Bilger, J Lejeune, V Béziat, V Rocha, B Rio, S Chevret, V Vieillard
    Bone Marrow Transplant., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Membrane-bound IL-21 promotes sustained ex vivo proliferation of human natural killer cells.
    Authors: Denman CJ, Senyukov VV, Somanchi SS
    PLoS ONE, 2012;7(1):e30264.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention.
    Authors: Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM
    J. Immunol., 0;186(1):62-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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