Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 387301
View all KOR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human KOR
Met1-Val380
Accession # P41145

Specificity

Detects human KOR. Stains human KOR transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human KOR Antibody

KOR in HEK293 Human Cell line Transfected with KOR.

KOR was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with KOR (positive staining) and HEK293 human embryonic kidney cell line (wild type, negative staining) using Mouse Anti-Human KOR Monoclonal Antibody (Catalog # MAB3895) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human KOR Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human KOR transfected HEK293 cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with KOR

Reviewed Applications

Read 1 review rated 5 using MAB3895 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KOR

KOR is a 45 kDa 7TM opioid receptor that is primarily expressed in the central nervous system and peripheral visceral pain sensory nerves. Following ligation by dynorphin peptides, KOR signaling induces analgesia, dysphoria, diuresis, and increased feeding desire. KOR also exerts neuroprotective and anti-inflammatory effects. Human KOR shares 94% amino acid sequence identity with mouse and rat KOR.

Long Name

Kappa Opioid Receptor

Alternate Names

OPRK1, Oprk2, R21

Entrez Gene IDs

4986 (Human); 18387 (Mouse); 29335 (Rat)

Gene Symbol

OPRK1

UniProt

P41145

Additional KOR Products

Product Documents for Human KOR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KOR Antibody

For research use only

Citations for Human KOR Antibody

Customer Reviews for Human KOR Antibody (1)

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  • Human KOR Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/28/2022
    Human KOR Antibody MAB3895

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Protocols

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FAQs

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