Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Kremen-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kremen‑1
Ala20-Gly397
Accession # NP_114434

Specificity

Detects human Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse Kremen-1 and less than 5% cross-reactivity with recombinant human Kremen-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Kremen‑1 Antibody

Kremen-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

Kremen‑1 in Human Colon Cancer Tissue.

Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Kremen-1 antibody in Human Colon by Immunohistochemistry (IHC-P).

Kremen‑1 in Human Colon.

Kremen-1 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2127) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Kremen‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013), and human colon array

Western Blot

0.1 µg/mL
Sample: Recombinant Human Kremen‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Kremen-1

Kremen-1 is a type I transmembrane protein that contains extracellular Kringle, WSC, and CUB domains. It is expressed in diverse embryonic and adult tissues. Kremen proteins bind the secreted Dickkopf proteins Dkk-1, -2, and -4 with high affinity to antagonize the canonical Wnt signaling pathway.

Long Name

Kringle-containing Transmembrane Protein 1

Alternate Names

Kremen1, KRM1

Entrez Gene IDs

83999 (Human); 84035 (Mouse)

Gene Symbol

KREMEN1

UniProt

NP_114434

Additional Kremen-1 Products

Product Documents for Human Kremen‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Kremen‑1 Antibody

For research use only

Related Research Areas

Wnt Family
Wnt Signaling Modulators

Citations for Human Kremen‑1 Antibody

Customer Reviews for Human Kremen‑1 Antibody

There are currently no reviews for this product. Be the first to review Human Kremen‑1 Antibody and earn rewards!

Have you used Human Kremen‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies