Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 762815
View all LEDGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LEDGF
Met1-Lys333
Accession # O75475

Specificity

Detects human LEDGF in direct ELISAs and Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LEDGF Antibody

Detection of Human LEDGF antibody by Western Blot.

Detection of Human LEDGF by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LEDGF antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

LEDGF in HeLa Human Cell Line.

LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LEDGF Monoclonal Antibody (Catalog # MAB3468) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LEDGF Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human HeLa cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB3468 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LEDGF

LEDGF, also known as transcription co-activator p75, is a 75 kDa protein that belongs to the hepatoma-derived growth factor (HDGF) family of nuclear targeted proteins. It is a widely expressed protein that interacts with lentiviral integrase. As a result of alternative splicing, isoforms with deletions, substitutions/insertions have been reported. Human LEDGF shares 91%, 92% and 96% amino acid sequence homology with rat, mouse and feline LEDGF, respectively.

Long Name

Lens Epithelium-derived Growth Factor

Alternate Names

CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52

Entrez Gene IDs

11168 (Human); 101739 (Mouse); 313323 (Rat)

Gene Symbol

PSIP1

UniProt

O75475

Additional LEDGF Products

Product Documents for Human LEDGF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LEDGF Antibody

For research use only

Citations for Human LEDGF Antibody

Customer Reviews for Human LEDGF Antibody (1)

5 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24040198
    Species: Mouse
    Verified Customer | Posted 02/20/2015

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Protocols

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