Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 748914
View all LGI2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LGI2
Gln29-Ser276
Accession # Q8N0V4

Specificity

Detects human LGI2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LGI2 Antibody

Detection of Human LGI2 antibody by Western Blot.

Detection of Human LGI2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, SH-SY5Y human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LGI2 Monoclonal Antibody (Catalog # MAB7334) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LGI2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LGI2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

LGI2 in HepG2 Human Cell Line.

LGI2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LGI2 Monoclonal Antibody (Catalog # MAB7334) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LGI2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample:

Immersion fixed HepG2 human hepatocellular carcinoma cell line

 

Western Blot

2 µg/mL
Sample: HEK293 human embryonic kidney cell line, SH‑SY5Y human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LGI2

LGI2 (leucine-rich, glioma-inactivated 2) is an approximately 100 kDa secreted glycoprotein in the LGI family of LRR (leucine-rich repeat) proteins. LGI2 cDNA encodes a 28 amino acid (aa) signal sequence and a 517 aa mature protein with 5 LRR domains and 7 EAR (epilepsy-associated repeat) domains. It is mainly expressed in various regions of the brain and spinal cord, but mRNA is also found in the heart and placenta. Mutation or deletion of LGI2 is associated with the temporal lobe epilepsy syndrome PEPS (partial epilepsy with pericentral spikes). Within aa 29-276, human LGI2 shares 99% aa sequence identity with mouse and rat LGI2, provided a 23 aa gap and a 32 aa insertion in mouse LGI2 at sites corresponding to human aa 139 and aa 218, respectively, are disregarded.

Long Name

Leucine-rich, Glioma-inactivated 2

Alternate Names

LGIL2

Entrez Gene IDs

55203 (Human); 246316 (Mouse); 305417 (Rat)

Gene Symbol

LGI2

UniProt

Q8N0V4 (Human)

Additional LGI2 Products

Product Documents for Human LGI2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LGI2 Antibody

For research use only

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Protocols

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