hh Human LIF R alpha Antibody AF-249-NA: R&D Systems

Human LIF R alpha Antibody

(11 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LIF R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 7% cross-reactivity with recombinant mouse LIF R alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LIF R alpha
    Gln45-Ser833
    Accession # P42702
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LIF R alpha (Catalog # 249-LR/CF)
  • Neutralization
    Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 6-36 µg/mL in the presence of 0.3 ng/mL Recombinant Human LIF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by LIF and Neutralization by Human LIF R alpha Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 0.3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-249-NA). The ND50 is typically 6-36 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF R alpha

The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha  subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

  • References:
    1. Bazan, J.F. (1990) Proc. Natl. Acad. Sci. USA 87:6934.
    2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
    3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.
  • Long Name:
    Leukemia Inhibitory Factor Receptor alpha
  • Entrez Gene IDs:
    3977 (Human); 16880 (Mouse); 81680 (Rat)
  • Alternate Names:
    CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The Phosphoinositide 3-Kinase p110alpha Isoform Regulates Leukemia Inhibitory Factor Receptor Expression via c-Myc and miR-125b to Promote Cell Proliferation in Medulloblastoma.
    Authors: Salm F, Dimitrova V, von Bueren A, Cwiek P, Rehrauer H, Djonov V, Anderle P, Arcaro A
    PLoS ONE, 2015;10(4):e0123958.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor.
    Authors: Chollangi, Srinivas, Mather, Timothy, Rodgers, Karla K, Ash, John D
    J Biol Chem, 2012;287(39):32848-59.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  3. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
    Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
    Fertil. Steril., 2009;91(6):2602-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Receptor-mediated transport of LIF across blood-spinal cord barrier is upregulated after spinal cord injury.
    Authors: Pan W, Cain C, Yu Y, Kastin AJ
    J. Neuroimmunol., 2006;174(1):119-25.
    Species: Mouse
    Sample Type:
    Application: Neut
  5. Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
    Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O
    Fertil. Steril., 2006;86(4):1193-209.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Breast cancer cells stimulate neutrophils to produce oncostatin M: potential implications for tumor progression.
    Authors: Queen MM, Ryan RE, Holzer RG, Keller-Peck CR, Jorcyk CL
    Cancer Res., 2005;65(19):8896-904.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Leukocytes, cytokines, growth factors and hormones in human skeletal muscle and blood after uphill or downhill running.
    Authors: Malm C, Sjodin TL, Sjoberg B, Lenkei R, Renstrom P, Lundberg IE, Ekblom B
    J. Physiol. (Lond.), 2004;556(0):983-1000.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  9. Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries.
    Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A
    Mol. Hum. Reprod., 2004;10(5):313-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  10. Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
    Authors: Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S
    J. Clin. Endocrinol. Metab., 2003;88(2):858-63.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Membrane distal cytokine binding domain of LIFR interacts with soluble CNTFR in vitro.
    Authors: He W, Gong K, Zhu G, Smith DK
    FEBS Lett., 2002;514(2):214-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 11 Citations
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