Detects human LILRA5/CD85f in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse LILRC1, recombinant rat LILRC1, recombinant human (rh) ILT7/LILRA4, rhILT4, rhILT5, or rhLILRB4/LIR5 is observed. In Western blots, no cross‑reactivity with rhILT7/LILRA4 or rhILT5 is observed.
Monoclonal Mouse IgG2A Clone # 711827
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f Gly42-Arg268 Accession # A6NI73
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB67541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILT11/LILRA5 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ILT11 (also leukocyte immunoglobulin-like receptor 9 (LIR-9), CD85f, and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human ILT11 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains (aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. ILT11 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41-268, human ILT11 shares 58% aa identity with mouse ILT11.
Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5
Entrez Gene IDs:
353514 (Human); 232801 (Mouse); 691533 (Rat)
CD85 antigen-like family member F; CD85; CD85f antigen; CD85f; ILT11; ILT-11; ILT11CD85f; Immunoglobulin-like transcript 11; leukocyte Ig-like receptor 9; Leukocyte immunoglobulin-like receptor 9; leukocyte immunoglobulin-like receptor subfamily A member 5 soluble; leukocyte immunoglobulin-like receptor subfamily A member 5; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRA5; LILRB7; LIR9; LIR-9; LIR9immunoglobulin-like transcript 11 protein; member 7
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