Human LPAR1/LPA₁/EDG-2 Antibody

Detection of Human LPAR1/LPA₁/EDG-2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human brain (cerebellum) tissue, and human liver tissue (negative control). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LPAR1/LPA₁/EDG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9963) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LPAR1/LPA₁/EDG-2 at approximately 48-51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of LPAR1/LPA1/EDG-2 by Western Blot miR-367 downregulates LPA1 expression. a Bioinformatics prediction of binding sites for miR-367 and LPA1. b Luminescence activity of LPA1 by dual luciferase reporter gene assay. c RIP assay detection of the binding of miR-367 and LPA1 to Ago2. d Detection of relative expression of LPA1 in cancer tissues and adjacent normal tissues by RT-qPCR. e Analysis of protein level of LPA1 in cancer and adjacent normal tissues by western blot analysis. f Statistical expression of protein level of LPA1. g Correlation analysis between miR-367 and LPA1. h RT-qPCR results of the relative expression level of LPA1 in each group of cells. i Western blot analysis of LPA1 protein levels in each group of cells. j Statistical protein expression level of LPA1. Measurement data were expressed as mean ± standard deviation. Data in compliance with normal distribution and homogeneity between two groups were compared using t test. Comparisons among multiple groups were conducted by one-way ANOVA followed by Tukey’s post hoc test. *p < 0.05 vs. normal tissues or cells; #p < 0.05 vs. Inhibitor NC The experiment was repeated three times independently Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33024414), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of LPAR1/LPA1/EDG-2 by Western Blot Low expression of LPA1 inhibits tumorigenic ability of ovarian cancer in nude mice. a Volume of mice tumor. b Weight of mice tumor. c RT-qPCR detection results of mRNA levels of related factors in tumor tissues. d Western blot analysis of the protein levels of related factors in tumor tissues. e Statistical graph of protein levels of related factors. f Immunohistochemistry for detecting CD34 expression (200×). Measurement data were expressed as mean ± standard deviation. Data in compliance with normal distribution and homogeneity between two groups were compared using t test. Comparisons among multiple groups were conducted by one-way ANOVA followed by Tukey’s post hoc test. Statistics of tumor volume at different time points was analyzed using repeated measures ANOVA. *p < 0.05 vs. normal tissues or cells. The experiment was repeated three times independently Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33024414), licensed under a CC-BY license. Not internally tested by R&D Systems.