Human LPAR1/LPA1/EDG-2 Antibody

Catalog # Availability Size / Price Qty
AF9963-100
AF9963-SP
Detection of Human LPAR1/LPA1/EDG-2 by Western Blot.
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Citations (1)
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Human LPAR1/LPA1/EDG-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LPAR-1/LPA1/EDG-2 in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LPAR-1 CD33 protein containing the sequences for the four extracellular domains, amino acids (aa) 1-50, aa112-125, aa190-205 and aa281-294
Accession # Q92633
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LPAR1/LPA<sub>1</sub>/EDG-2 antibody by Western Blot. View Larger

Detection of Human LPAR1/LPA1/EDG-2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human brain (cerebellum) tissue, and human liver tissue (negative control). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LPAR1/LPA1/EDG-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9963) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LPAR1/LPA1/EDG-2 at approximately 48-51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR1/LPA1/EDG-2

Lysophosphatidic acid receptor 1, LPAR1, also known as EDG-2, is a G protein-coupled receptor that binds the lipid signaling molecule lysophosphatidic acid (LPA). EDG molecules are G protein-coupled receptors that bind plasma lysophospholipids. The EDG family consists of two subfamilies; the S1P (sphingosine-1-phosphate) subfamily consisting of EDG-1, 3, 5, 6, and 8, and the LPA subfamily that contains EDG-2, 4 and 7. The S1P family regulates essential cellular processes such as proliferation, migration, cytoskeletal organization, and differentiation.

Long Name
Lysophosphatidic Acid Receptor 1
Entrez Gene IDs
1902 (Human); 14745 (Mouse)
Alternate Names
EDG2; edg-2; EDG2Lysophosphatidic acid receptor Edg-2; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor2,; GPCR26; GPR26; LPA receptor 1; LPA1; LPA-1; LPA1vzg-1; LPAR1; lysophosphatidic acid receptor 1; Mrec1.3; rec.1.3; ventricular zone gene 1; VZG1; vzg-1

Product Datasheets

Citation for Human LPAR1/LPA1/EDG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
    Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
    Cancer Cell Int, 2020;20(0):476.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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