Human LRP-6 Antibody

Catalog # Availability Size / Price Qty
AF1505
AF1505-SP
Human LRP-6 Antibody in Western Blot
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human LRP-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRP-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse LRP-6 is observed, and less than 1% cross-reactivity with recombinant human (rh) LRP-1, rhLRP-4, and rhLRP-5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRP-6
Ala20-Pro1368
Accession # O75581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
HEK293 human embryonic kidney cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRP‑6 by Western Blot. View Larger

Detection of Human LRP‑6 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with human LRP‑6. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human LRP‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1505) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LRP‑6 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family. Both LRP-5 and LRP-6 are type I transmembrane proteins that function as co-receptors with Frizzled (FZD) in the canonical Wnt signaling pathway (1, 2). LRP-6 cDNA encodes a 1613 amino acid residue (aa) precursor with a 19 aa signal sequence, a 1353 aa extracellular region, a 23 aa transmembrane (TM) segment and a 218 aa cytoplasmic domain (3). The extracellular region contains four N-terminal cysteine-rich EGF-like repeats, followed by three cysteine-rich LDLR repeats. This pattern of the EGF and LDLR repeat arrangement is different than that typically found in other LDL receptor family proteins. The intracellular region of LRP6 contains protein-protein interaction motifs and is required for canonical Wnt signal transduction (4). Human LRP-6 shares 98% and 74% aa sequence identity with mouse LRP-6 and human LRP-5, respectively. Based on the current model, canonical Wnt signaling requires the interaction of Wnt with FZD and LRP to form a trimeric complex which signals downstream to stabilize cytoplasmic  beta -catenin. The stabilized beta -catenin is then translocated to the nucleus where it complexes with the transcription factor LEF/TCF to regulate the transcription of target genes (5). LRP-6 has also been shown to interact with the Dickkopf proteins (DKKs), which are modulators of Wnt signaling (6-8). Binding of DKK-1 to LRP-6 dissociates LRP-6 from FZD, and antagonizes the formation of the functional receptor complex. On cells where the transmembrane proteins Kremens are also present, a ternary complex of LRP-6, DKK-1 and Kremen is formed to trigger the internalization of the complex and removal LRP6 from the cell surface. Thus, DKK-1 and Kremen function synergistically to antagonize LRP-6-mediated Wnt activity. Although DKK-2 also functions as a Wnt antagonist on cells that express Kremen, DKK-2 binding to LRP-6 in the absence of Kremen activates rather than inhibits LRP mediated beta -catenin signaling (9, 10).

References
  1. Howell, B.W. and J. Herz (2001) Curr. Op. Neurobiology 11:74.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. Tamai, K. et al. (2000) Nature 407:530.
  5. Schweizer, L. and H. Varmus (2003) BMC Cell Biology 4:4.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  8. Bafico, A. et al. (2001) Nature Cell Biol. 3:683.
  9. Zorn, A.M. (2001) Curr. Biol. 11:R592.
  10. Mao, B. et al. (2002) Nature 417:664.
Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

Product Datasheets

Citations for Human LRP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. A novel high-content imaging-based technique for measuring binding of Dickkopf-1 to low-density lipoprotein receptor-related protein 6
    Authors: RS Priestley, J Cheung, EJ Murphy, MT Ehebauer, JB Davis, E Di Daniel
    J Pharmacol Toxicol Methods, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. The Novel Secreted Adipokine WNT1-inducible Signaling Pathway Protein 2 (WISP2) Is a Mesenchymal Cell Activator of Canonical WNT.
    Authors: Grunberg J, Hammarstedt A, Hedjazifar S, Smith U
    J Biol Chem, 2014;289(10):6899-907.
  3. Activation of canonical wingless-type MMTV integration site family (Wnt) signaling in mature adipocytes increases beta-catenin levels and leads to cell dedifferentiation and insulin resistance.
    Authors: Gustafson B, Smith U
    J. Biol. Chem., 2010;285(18):14031-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Cooperative folding and ligand-binding properties of LRP6 beta-propeller domains.
    Authors: Liu CC, Pearson C, Bu G
    J. Biol. Chem., 2009;284(22):15299-307.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human LRP-6 Antibody

There are currently no reviews for this product. Be the first to review Human LRP-6 Antibody and earn rewards!

Have you used Human LRP-6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review