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Human Lumican Antibody

Catalog #: AF2846
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2846
AF2846-SP

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Detection of Human Lumican by Western Blot.
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Citations (15)
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Human Lumican Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lumican in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse Lumican is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # P51884
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
Human colon tissue
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lumican antibody by Western Blot. View Larger

Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lumican antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Lumican in Human Kidney. Lumican was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human Lumican by Simple WesternTM. Simple Western shows lysates of human colon tissue, loaded at 0.5 mg/ml. A specific band was detected for Lumican at approximately 100-180 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain. The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3‑6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8, 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).

References
  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294.
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.
Entrez Gene IDs
4060 (Human); 17022 (Mouse)
Alternate Names
KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

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Citations for Human Lumican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Extracellular lumican augments cytotoxicity of chemotherapy in pancreatic ductal adenocarcinoma cells via autophagy inhibition
    Authors: X Li, D Roife, Y Kang, B Dai, M Pratt, J B Fleming
    Oncogene
  2. Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity
    Authors: Daniella dos Santos Courrol, Cristiane Castilho Fernandes da da Silva, Luan Gavião Prado, Rosa Maria Chura-Chambi, Ligia Morganti, Gisele Oliveira de Souza et al.
    Frontiers in Cellular and Infection Microbiology
  3. Lumican deficiency results in cardiomyocyte hypertrophy with altered collagen assembly
    Authors: Loren E. Dupuis, Matthew G. Berger, Samuel Feldman, Lorna Doucette, Vennece Fowlkes, Shukti Chakravarti et al.
    Journal of Molecular and Cellular Cardiology
  4. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering
    Authors: Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al.
    Frontiers in Bioengineering and Biotechnology
  5. Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering
    Authors: Blanco-Elices C, Morales-�lvarez C, Chato-Astrain J et al.
    Frontiers in Bioengineering and Biotechnology
  6. Identifying new biomarkers of aggressive Group�3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging
    Authors: F Linke, JEC Johnson, S Kern, CD Bennett, A Lourdusamy, D Lea, SC Clifford, CLR Merry, S Stolnik, MR Alexander, AC Peet, DJ Scurr, RL Griffiths, AM Grabowska, ID Kerr, B Coyle
    Acta neuropathologica communications, 2023-01-11;11(1):6.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  7. Hypoxia-induced autophagy of stellate cells inhibits expression and secretion of lumican into microenvironment of pancreatic ductal adenocarcinoma
    Authors: X Li, Y Lee, Y Kang, B Dai, MR Perez, M Pratt, EJ Koay, M Kim, RA Brekken, JB Fleming
    Cell Death Differ., 2018-10-03;26(2):382-393.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Extracellular lumican inhibits pancreatic cancer cell growth and is associated with prolonged survival after surgery.
    Authors: Li X, Truty M, Kang Y, Chopin-Laly X, Zhang R, Roife D, Chatterjee D, Lin E, Thomas R, Wang H, Katz M, Fleming J
    Clin Cancer Res, 2014-10-21;20(24):6529-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
    Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
    Invest Ophthalmol Vis Sci, 2013-02-05;54(2):1014-25.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  10. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
    Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
    Lab Invest, 2012-09-24;92(12):1712-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
    Authors: Zhang Y, Conrad AH, Conrad GW
    J. Biol. Chem., 2011-02-18;286(15):13011-22.
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. HMGA2 overexpression-induced ovarian surface epithelial transformation is mediated through regulation of EMT genes.
    Authors: Wu J, Liu Z, Shao C, Gong Y, Hernando E, Lee P, Narita M, Muller W, Liu J, Wei JJ
    Cancer Res., 2011-01-11;71(2):349-59.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Vascular Endothelial Growth Factor (VEGF) Induced Downstream Responses to Transient Receptor Potential Vanilloid 1 (TRPV1) and 3-Iodothyronamine (3-T1AM) in Human Corneal Keratocytes
    Authors: Ersal Türker, Fabian Garreis, Noushafarin Khajavi, Peter S. Reinach, Pooja Joshi, Tobias Brockmann et al.
    Front Endocrinol (Lausanne)
  14. The Downregulation of Placental Lumican Promotes the Progression of Preeclampsia
    Authors: Chao Liu, Yulian Hu, Zhongying Wang, Hua Pan, Yan Ren, Xiao Li et al.
    Reproductive Sciences
  15. Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with fibroblast growth factor-2, transforming growth factor-beta 3 and retinoic acid
    Authors: Laura E. Sidney, Andrew Hopkinson
    Journal of Tissue Engineering and Regenerative Medicine

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