Human Lumican Antibody

AF2846 6 Citations Datasheet / COA / SDS

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AF2846
AF2846-SP

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Detection of Human Lumican by Western Blot.

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Citations (6)

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Human Lumican Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lumican in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse Lumican is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # P51884

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human Lumican by Western Blot. Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lumican at approximately 60-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Lumican in Human Kidney. Lumican was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Lumican Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2846) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain. The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3‑6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8, 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).

References

Nikitovic, D. et al. (2008) IUBMB Life 60:818.
Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294.
Nikitovic, D. et al. (2008) FEBS J. 275:350.
D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Alternate Names

KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican

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Citations for Human Lumican Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Hypoxia-induced autophagy of stellate cells inhibits expression and secretion of lumican into microenvironment of pancreatic ductal adenocarcinoma
Authors: X Li, Y Lee, Y Kang, B Dai, MR Perez, M Pratt, EJ Koay, M Kim, RA Brekken, JB Fleming
Cell Death Differ., 2019;26(2):382-393.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Extracellular lumican inhibits pancreatic cancer cell growth and is associated with prolonged survival after surgery.
Authors: Li X, Truty M, Kang Y, Chopin-Laly X, Zhang R, Roife D, Chatterjee D, Lin E, Thomas R, Wang H, Katz M, Fleming J
Clin Cancer Res, 2014;20(24):6529-40.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
Invest Ophthalmol Vis Sci, 2013;54(2):1014-25.
Species: Human
Sample Types: Protein
Applications: Western Blot
Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
Lab Invest, 2012;92(12):1712-25.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
Authors: Zhang Y, Conrad AH, Conrad GW
J. Biol. Chem., 2011;286(15):13011-22.
Species: Bovine
Sample Types: Tissue Homogenates
Applications: Western Blot
HMGA2 overexpression-induced ovarian surface epithelial transformation is mediated through regulation of EMT genes.
Authors: Wu J, Liu Z, Shao C, Gong Y, Hernando E, Lee P, Narita M, Muller W, Liu J, Wei JJ
Cancer Res., 2011;71(2):349-59.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot