Human MafG Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MafG in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafF or rhMafK is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 370412
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human MafG
    Met1-Ser162
    Accession # O15525
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MafG by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human MafG by Western Blot. Western blot shows recombinant human MafF, MafG, and MafK (5 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human MafG Monoclonal Antibody (Catalog # MAB3924) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for MafG was detected at approximately 18 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MafG

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members.

  • Long Name:
    v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog G
  • Entrez Gene IDs:
    4097 (Human); 17134 (Mouse); 64188 (Rat)
  • Alternate Names:
    basic leucine zipper transcription factor MafG; hMAF; MafG; MGC13090; MGC20149; transcription factor MafG; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G; v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian); V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
Related Research Areas
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse IgG2A Antibody

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Rat Anti-Mouse IgG2A APC-conjugated Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

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