Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MBD3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MBD3 isoform 2
Met1-Val259
Accession # AAH43619

Specificity

Detects human MBD3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human MBD3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MBD3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MBD3

MBD3 (methyl-CpG-binding domain protein # 3) is a 38‑40 kDa member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD2. Human MBD3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa’s 1‑72) and a C-terminal coiled-coil region (aa’s 216‑245) that may be involved in protein-protein interaction. Human MBD3 shares 95% aa identity with mouse, rat, and canine MBD3, with differences essentially limited to the C‑terminal 25 amino acids.

Long Name

Methyl-CpG Binding Domain Protein 3

Alternate Names

methyl-CpG binding domain protein 3, methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3

Entrez Gene IDs

53615 (Human); 17192 (Mouse); 314628 (Rat)

Gene Symbol

MBD3

UniProt

AAH43619

Additional MBD3 Products

Product Documents for Human MBD3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MBD3 Antibody

For research use only

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