Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry, Antibody Profiling, Dynamic Light Scattering

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 128018
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCAM
Val24-Gly559
Accession # AAA20824

Specificity

Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no  cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MCAM/CD146 Antibody

Detection of Human MCAM/CD146 antibody by Western Blot.

Detection of Human MCAM/CD146 by Western Blot.

Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MCAM/CD146 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
MCAM/CD146 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

MCAM/CD146 in BG01V Human Embryonic Stem Cells.

MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of MCAM/CD146 by Western Blot

Detection of MCAM/CD146 by Western Blot

MCAM and PECAM are both involved in PNA-mediated cytokine secretion. The presence of ASF or neutralizing antibodies to MCAM or PECAM reduced PNA-mediated secretion of MCP-1 and IL-6 (A) from HUVECs. siRNA suppression of MCAM and PECAM expression (B) each abolished PNA-induced secretion of MCP-1 and IL-6 (C). Data are presented as Mean ± SD of three independent experiments, each in triplicate. **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34223877), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MCAM/CD146 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCAM/CD146

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

AAA20824

Additional MCAM/CD146 Products

Product Documents for Human MCAM/CD146 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MCAM/CD146 Antibody

For research use only

Citations for Human MCAM/CD146 Antibody

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Protocols

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FAQs

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