Human MCAM/CD146 Antibody

Catalog #: MAB932
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB932-SP
MAB932-500
MAB932-100

Request a Quote Bulk Orders

Detection of Human MCAM/CD146 by Western Blot.

3 Images

Product Details

Citations (8)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MCAM/CD146 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 128018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MCAM
Val24-Gly559
Accession # AAA20824

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human MCAM/CD146 by Western Blot. Western blot shows lysate of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCAM/CD146 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

View Larger

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

View Larger

MCAM/CD146 in BG01V Human Embryonic Stem Cells. MCAM/CD146 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human MCAM/CD146 Monoclonal Antibody (Catalog # MAB932) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCAM/CD146

MCAM, also known as MUC18, is a putative adhesion molecule that belongs to the immunoglobulin superfamily. It is associated with tumor progression and metastasis of various carcinomas and may be involved in embryonic neural development.

Long Name

Melanoma Cell Adhesion Molecule

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Alternate Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human MCAM/CD146 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

Proteomics profiling identifies extracellular vesicles' cargo associated with tumour cell induced platelet aggregation
Authors: N McNamee, LR de la Fuen, MJ Santos-Mar, L O'Driscoll
BMC Cancer, 2022;22(1):1023.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
Sci Adv, 2020;6(9):eaax3223.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems
Authors: J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau
J Colloid Interface Sci, 2019;537(0):536-546.
Species: Human
Sample Types: Recombinant Protein
Applications: Dynamic Light Scattering
Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
Mol Med Rep, 2019;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry
Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles
Authors: MP Zaborowski, K Lee, YJ Na, A Sammarco, X Zhang, M Iwanicki, PS Cheah, HY Lin, M Zinter, CY Chou, G Fulci, BA Tannous, CP Lai, MJ Birrer, R Weissleder, H Lee, XO Breakefiel
Cell Rep, 2019;27(1):255-268.e6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Stem Cells Int, 2016;2016(0):6104780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor.
Authors: Im, Hyungsoo, Shao, Huilin, Park, Yong Il, Peterson, Vanessa, Castro, Cesar M, Weissleder, Ralph, Lee, Hakho
Nat Biotechnol, 2014;32(5):490-5.
Species: N/A
Sample Types: Cell Lysates
Applications: Antibody Profiling
Choice of xenogenic-free expansion media significantly influences the myogenic differentiation potential of human bone marrow-derived mesenchymal stromal cells.
Authors: Brun J, Abruzzese T, Rolauffs B, Aicher W, Hart M
Cytotherapy, 0;18(3):344-59.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry