Detection of Human MDL‑1/CLEC5A by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MDL‑1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MDL‑1/CLEC5A at approximately 37-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MDL‑1/CLEC5A in Human Joint.
MDL‑1/CLEC5A was detected in formalin fixed paraffin-embedded sections of human rheumatoid arthritis joint using Goat Anti-Human MDL‑1/CLEC5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2384) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of monocyte colony cells in rheumatoid arthritis joint. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MDL-1 is an
approximately 40 kDa transmembrane glycoprotein belonging to the C-type lectin
superfamily (CLEC5A). MDL-1 is expressed on immature myeloid cells, monocytes,
macrophages, dendritic cells, neutrophils, NK cells, and osteocytes. It
contains a charged lysine in the transmembrane region that enables it to
associate with DAP12 and deliver an activating signal. MDL-1 mediates inflammatory responses during
autoimmune arthritis and upon binding to Dengue and Japanese encephalitis viruses.
The extracellular domain (ECD) of MDL-1 contains a juxtamembrane stalk region
and one C-type lectin domain. Within the ECD, human and mouse MDL-1 share 67%
amino acid sequence identity.
Myeloid DAP12-associating Lectin
Entrez Gene IDs:
23601 (Human); 23845 (Mouse)
CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1
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