Human Megalin/LRP2 Antibody

Catalog # Availability Size / Price Qty
MAB9578-100
MAB9578-SP

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Detection of Megalin/LRP2 in CaCo-2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Reviews (1)

Human Megalin/LRP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Megalin/LRP2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 545606
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Megalin/LRP2
Pro3510-Lys3964
Accession # P98164
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Megalin/LRP2 antibody in CaCo-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Megalin/LRP2 in CaCo-2 Human Cell Line by Flow Cytometry. CaCo2 human cell line was stained with Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB9578, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Megalin/LRP2 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Megalin/LRP2 in Human Kidney. Megalin/LRP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB9578) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Megalin/LRP2

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi‑ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the re-uptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N‑terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C‑terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4.

References
  1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
  2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
  3. Kantarci, S. et al. (2007) Nat. Genet 39:957.
Long Name
LDL Receptor-related Protein 2
Entrez Gene IDs
4036 (Human); 14725 (Mouse); 29216 (Rat)
Alternate Names
calcium sensor protein; DBS; DBSlow-density lipoprotein receptor-related protein 2; EC 1.1.2.3; EC 3.4.21.9; Glycoprotein 330; gp330; Heymann nephritis antigen homolog; low density lipoprotein receptor-related protein 2; LRP2; LRP-2; Megalin

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Citation for Human Megalin/LRP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation
    Authors: C Jacobs-Cac, N Puig-Gay, A Vergara, MA Gabaldon, J Sellarés, Y Villena-Or, I Agraz, F Moreso, MJ Soler, D Serón, J López-Hell
    Journal of Clinical Medicine, 2021;10(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human Megalin/LRP2 Antibody
By Anonymous on 11/20/2021
Application: Immunohistochemistry Sample Tested: Kidney tissue Species: Human