Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human Melusin
Ser2-Glu347
Accession # Q9UKP3

Specificity

Detects human Melusin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Melusin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skeletal muscle

Western Blot

0.1 µg/mL
Sample: Recombinant Human Melusin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Melusin

Melusin is a 38 kDa cytoplasmic protein that interacts specifically with the cytoplasmic domain of the beta 1 integrin subunit. It is expressed solely in striated muscle where it detects mechanical stress. The 347 amino acid residue human Melusin contains two amino terminal cysteine-rich repeats that are separated by an intervening sequence at the extreme carboxy-terminal. Melusin has a stretch of negatively charged residues (aspartic and glutamic acids). Human Melusin shares 93%, 92% and 96% amino acid sequence homology with rat, mouse and canine Melusin, respectively.

Alternate Names

CHORDC3, ITGB1BP2

Entrez Gene IDs

26548 (Human)

Gene Symbol

ITGB1BP2

UniProt

Additional Melusin Products

Product Documents for Human Melusin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Melusin Antibody

For research use only

Related Research Areas

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Protocols

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