Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2030B
View all MESP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-dervived recombinant human MESP1
Met1-Gln85
Accession # Q9BRJ9

Specificity

Detects human MESP1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human MESP1 Antibody

Detection of Human MESP1 antibody by Western Blot.

Detection of Human MESP1 by Western Blot.

Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.
Detection of Human MESP1 antibody by Western Blot.

Detection of Human MESP1 by Western Blot.

Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.
Human MESP1 antibody in H1 Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Human MESP1 in H1 Human Embryonic Stem Cells.

MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Applications for Human MESP1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Fixed H1 human embryonic stem cells treated with Doxycycline

Western Blot

2 µg/mL
Sample: Mouse ES cells transfected with human MESP1 treated with Doxycycline and H1 human embryonic stem cells treated with Doxycycline

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MESP1

Mesoderm posterior protein 1 (MESP1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. MESP1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that MESP1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Long Name

Mesoderm Posterior BHLH Transcription Factor 1

Alternate Names

bHLHc5

Entrez Gene IDs

55897 (Human); 17292 (Mouse); 308766 (Rat)

Gene Symbol

MESP1

UniProt

Q9BRJ9

Additional MESP1 Products

Product Documents for Human MESP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MESP1 Antibody

For research use only

Citations for Human MESP1 Antibody

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Protocols

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