Human MESP1 Antibody

Catalog #: MAB9219 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9219-SP
MAB9219-100

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Detection of Human MESP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MESP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MESP1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2030B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-dervived recombinant human MESP1
Met1-Gln85
Accession # Q9BRJ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MESP1 by Western Blot. Western blot shows lysates of Mouse ES cells transfected with human MESP1 untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. Cells provided courtesy of Michael Kyba's lab, University of Minnesota.

Western Blot

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Detection of Human MESP1 by Western Blot. Western blot shows lysates of H1 human embryonic stem cells untreated (-) or treated (+) with 100 ng/mL Doxycycline overnight. PVDF membrane was probed with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MESP1 at approximately 30 kDa (as indicated). Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Immunocytochemistry

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Human MESP1 in H1 Human Embryonic Stem Cells. MESP1 was detected in fixed H1 human embryonic stem cells untreated (lower panel) or treated (upper panel) with Doxycycline using Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219). Cells were stained using anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).Image provided courtesy of Michael Kyba's lab, University of Minnesota.

Intracellular Staining by Flow Cytometry

Detection of MESP1 antibody in Mouse ES cells transfected with human MESP1 antibody by Flow Cytometry.

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Detection of MESP1 in Mouse ES cells transfected with human MESP1 by Flow Cytometry. Mouse ES cells transfected with human MESP1 were stained with Rabbit Anti-Human MESP1 Monoclonal Antibody (Catalog # MAB9219, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MESP1

Mesoderm posterior protein 1 (MESP1) is a 268 amino acids protein that in humans is encoded by the MESP1 gene. MESP1 was first identified in transcripts enriched in the posterior region of the mouse embryo at embryonic day E7 to E7.5. Lineage tracing in mice showed that MESP1 represents the earliest marker of cardiac progenitors and directs multipotential cardiovascular cell fates, patterning mesoderm into cardiac, hematopoietic, or skeletal myogenic progenitors in a context-dependent manner.

Long Name

Mesoderm Posterior BHLH Transcription Factor 1

Entrez Gene IDs

55897 (Human); 17292 (Mouse); 308766 (Rat)

Alternate Names

bHLHc5; bHLHc5MGC10676; Class C basic helix-loop-helix protein 5; mesoderm posterior 1 homolog (mouse); mesoderm posterior protein 1; MESP1