Human Mineralocorticoid R/NR3C2 Antibody

Catalog # Availability Size / Price Qty
MAB4369
MAB4369-SP
Human Mineralocorticoid R/NR3C2 Antibody in Western Blot
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Human Mineralocorticoid R/NR3C2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mineralocorticoid R/NR3C2.
Source
Monoclonal Mouse IgG2B Clone # 385707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Mineralocorticoid R/NR3C2
Met1-Leu670
Accession # AAI11759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human prostate cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Mineralocorticoid R/NR3C2 by Western Blot. View Larger

Detection of Human Mineralocorticoid R/NR3C2 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Mineralocorticoid R/NR3C2 Monoclonal Antibody (Catalog # MAB4369) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mineralocorticoid R/NR3C2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mineralocorticoid R/NR3C2

Mineralocorticoid Receptor (MR) is a member of the nuclear hormone receptor family. Upon binding of ligand (aldosterone or corticosterone) MR translocates to the nucleus and can form either homo or heterodimers. MR dimers bind to the mineralocorticoid response element (AGAACANNNTGTTCT) to regulate gene transcription. There are four known isoforms of MR.

Long Name
Mineralocorticoid Receptor
Entrez Gene IDs
4306 (Human)
Alternate Names
FLJ41052; MGC133092; Mineralocorticoid R; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MineralocorticoidR; MLR; MLRmineralocorticoid receptor; MRMCRaldosterone receptor; NR3C2; NR3C2VIT; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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