Human Mineralocorticoid R/NR3C2 Antibody

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Human Mineralocorticoid R/NR3C2 Antibody Summary

Species Reactivity
This antibody specifically recognizes human MR and cross-reacts with mouse and rat MR. Not yet tested in other species.
Monoclonal Mouse IgG2A Clone # H3122
Protein A or G purified from hybridoma culture supernatant
Recombinant human Mineralocorticoid R/NR3C2
aa 2-99
A liquid formulation in physiologic saline with 0.1% NaN3.


Recommended Concentration
Western Blot
This antibody can be used at 1 μg/mL under reducingconditions with the appropriate secondary reagents to detect human MR.
This antibody can be used at 20 - 40 μg/mL withthe appropriate secondary reagents to detect human MR.
Optimal dilutions should be determined by eachlaboratory.
Direct ELISA
This antibody can be used at 0.2 μg/mL with the appropriatesecondary reagents to detect human MR.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: Mineralocorticoid R/NR3C2

Mineralcorticoid Receptor (MR; NR3C2) is a member of the Steroid Receptor superfamily (AR,ER,GR,PR). Aldosterone binds to MR with a high affinity. MR is expressed in the kidney, gut, brain, pituitary, hypothalamus and heart. MR plays a critical role in regulating electrolyte and fluid balance in the kidney, as well as having specific roles in the central nervous system. The protein is expressed as a 107 kDa nuclear form. MR and GR can cooperatively form heterodimers.

Long Name
Mineralocorticoid Receptor
Entrez Gene IDs
4306 (Human)
Alternate Names
FLJ41052; MGC133092; Mineralocorticoid R; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MineralocorticoidR; MLR; MLRmineralocorticoid receptor; MRMCRaldosterone receptor; NR3C2; NR3C2VIT; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2

Product Datasheets


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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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