Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation, Immunoaffinity Purification

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 82902
View all MMP-12 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP-12

Specificity

Detects human MMP‑12 Catalytic Domain. Does not recognize the C-terminal hemopexin-like domain of human MMP-12. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -13.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MMP-12 Catalytic Domain Antibody (82902)

Detection of MMP-12 Catalytic Domain by Immunohistochemistry

Detection of MMP-12 Catalytic Domain by Immunohistochemistry

MMP-12 expression level becomes increasingly elevated as prostate cancer progresses: A, The MMP-12 protein expression (left) and quantification (right) in patients with pathological stages of benign prostatic hyperplasia (BPH), androgen-dependent prostate cancer (ADPC), and castration-resistant prostate cancer (CRPC) was determined by immunohistochemistry (scale bar 100 μm). B, The concentration of MMP-12 protein in the serum of the different groups of patients was determined by ELISA. Data are reported as means±SD. **P<0.01, ***P<0.001, and ****P<0.0001 unpaired t-test. ns: not significant. Image collected and cropped by CiteAb from the following open publication (http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X202400…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-12 Catalytic Domain Antibody (82902)

Application
Recommended Usage

Immunoaffinity Purification

Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-12 (Catalog # 917-MP) from conditioned cell culture media.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑12 (Catalog # 917‑MP), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑12 Western Blot Standard (Catalog # WBC019)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-12

MMP-12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. The MMP-12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Entrez Gene IDs

4321 (Human); 17381 (Mouse)

Gene Symbol

MMP12

Additional MMP-12 Products

Product Documents for Human MMP-12 Catalytic Domain Antibody (82902)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-12 Catalytic Domain Antibody (82902)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP-12 Catalytic Domain Antibody (82902)

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Protocols

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FAQs

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