Human MMP-12 Catalytic Domain Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (8)
Supplemental Products

Human MMP-12 Catalytic Domain Antibody Summary

Species Reactivity
Detects human MMP‑12 Catalytic Domain. Does not recognize the C-terminal hemopexin-like domain of human MMP-12. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -13.
Monoclonal Mouse IgG2B Clone # 82902
Protein A or G purified from ascites
Chinese hamster ovary cell line CHO-derived recombinant human MMP-12
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human MMP‑12 Western Blot Standard (Catalog # WBC019)
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑12 (Catalog # 917‑MP), see our available Western blot detection antibodies
Immunoaffinity Purification
Sepharose beads coupled with this antibody have been used to immunoprecipitate and immunopurify Recombinant Human MMP-12 (Catalog # 917-MP) from conditioned cell culture media.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-12

MMP-12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. The MMP-12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 12
Entrez Gene IDs
4321 (Human); 17381 (Mouse)
Alternate Names
EC 3.4.24; hME; HMEEC; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12

Product Datasheets

You must select a language.


Citations for Human MMP-12 Catalytic Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Comprehensive Gene Analysis of IgG4-Related Ophthalmic Disease Using RNA Sequencing
    Authors: M Asakage, Y Usui, N Nezu, H Shimizu, K Tsubota, K Umazume, N Yamakawa, T Umezu, H Suwanai, M Kuroda, H Goto
    J Clin Med, 2020;9(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
    Authors: Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby
    PLoS ONE, 2016;0(0):1932-6203.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Fluid shear promotes chondrosarcoma cell invasion by activating matrix metalloproteinase 12 via IGF-2 and VEGF signaling pathways.
    Authors: Wang P, Chen S, Hung W, Paul C, Zhu F, Guan P, Huso D, Kontrogianni-Konstantopou A, Konstantopoulos K
    Oncogene, 2015;34(35):4558-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2009;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  5. Macrophage-specific metalloelastase (MMP-12) truncates and inactivates ELR+ CXC chemokines and generates CCL2, -7, -8, and -13 antagonists: potential role of the macrophage in terminating polymorphonuclear leukocyte influx.
    Authors: Dean RA, Cox JH, Bellac CL, Doucet A, Starr AE, Overall CM
    Blood, 2008;112(8):3455-64.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  6. Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
    Authors: Coury F, Annels N, Rivollier A, Olsson S, Santoro A, Speziani C, Azocar O, Flacher M, Djebali S, Tebib J, Brytting M, Egeler RM, Rabourdin-Combe C, Henter JI, Arico M, Delprat C
    Nat. Med., 2007;14(1):81-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
    Authors: Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, Van Den Berg A
    J. Pathol., 2006;209(4):474-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. Human bronchial epithelial cells express and secrete MMP-12.
    Authors: Lavigne MC, Thakker P, Gunn J, Miyashiro JS, Wasserman AM, Wei SQ, Pelker JW
    Biochem. Biophys. Res. Commun., 2004;324(2):534-46.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-12 Catalytic Domain Antibody

There are currently no reviews for this product. Be the first to review Human MMP-12 Catalytic Domain Antibody and earn rewards!

Have you used Human MMP-12 Catalytic Domain Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review