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Human MMP-14/MT1-MMP Antibody

Catalog #: MAB918
3 Citations Datasheet / COA / SDS
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MAB918
MAB918-SP

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Detection of Human MMP‑14/MT1‑MMP by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-14/MT1-MMP Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human (rh) MMP-14 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -7,
-8, -9, -10, -12, -13 or the catalytic domains of rhMMP-15 and -16.
Source
Monoclonal Mouse IgG1 Clone # 5H2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human MMP-14 synthetic peptide
AYIREGHEKQA
Accession # P50281
Formulation
Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑14 (Catalog # 918‑MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MMP-14/MT1-MMP antibody by Western Blot. View Larger

Detection of Human MMP‑14/MT1‑MMP by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP‑14 antibody in Human Benign Nodular Hyperplasia by Immunohistochemistry (IHC-P). View Larger

MMP‑14 in Human Benign Nodular Hyperplasia. MMP-14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP-14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-14/MT1-MMP

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and Integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2).

References
  1. Seike, M. (2003) Cancer Lett. 194:1.
  2. Sato, H. et al. (1994) Nature 370:61.
Long Name
Matrix Metalloproteinase 14/Membrane Type 1 MMP
Entrez Gene IDs
4323 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1

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Citations for Human MMP-14/MT1-MMP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Selective blockade of matrix metalloprotease-14 with a monoclonal antibody abrogates invasion, angiogenesis, and tumor growth in ovarian cancer.
    Authors: Kaimal R, Aljumaily R, Tressel S, Pradhan R, Covic L, Kuliopulos A, Zarwan C, Kim Y, Sharifi S, Agarwal A
    Cancer Res, 2013-02-19;73(8):2457-67.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  2. Laminin-111 derived peptides AG73 and C16 regulate invadopodia activity of a human adenoid cystic carcinoma cell line.
    Authors: Nascimento C, de Siqueira A, Pinheiro J, Freitas V, Jaeger R
    Exp Cell Res, 2011-09-07;317(18):2562-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Hyaluronic acid and thrombin upregulate MT1-MMP through PI3K and Rac-1 signaling and prime the homing-related responses of cord blood hematopoietic stem/progenitor cells.
    Authors: Shirvaikar N, Marquez-Curtis LA, Ratajczak MZ, Janowska-Wieczorek A
    Stem Cells Dev., 2010-10-25;20(0):19-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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