Human MMP-14/MT1-MMP Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human (rh) MMP-14 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP-1, -2, -7,
    -8, -9, -10, -12, -13 or the catalytic domains of rhMMP-15 and -16.
  • Source
    Monoclonal Mouse IgG1 Clone # 5H2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human MMP-14 synthetic peptide
    AYIREGHEKQA
    Accession # P50281
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
MMP‑14 in Human Benign Nodular Hyperplasia. MMP‑14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP‑14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-14/MT1-MMP

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and Integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2).

  • References:
    1. Seike, M. (2003) Cancer Lett. 194:1.
    2. Sato, H. et al. (1994) Nature 370:61.
  • Long Name:
    Matrix Metalloproteinase 14/Membrane Type 1 MMP
  • Entrez Gene IDs:
    4323 (Human)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Selective blockade of matrix metalloprotease-14 with a monoclonal antibody abrogates invasion, angiogenesis, and tumor growth in ovarian cancer.
    Authors: Kaimal R, Aljumaily R, Tressel S, Pradhan R, Covic L, Kuliopulos A, Zarwan C, Kim Y, Sharifi S, Agarwal A
    Cancer Res, 2013;73(8):2457-67.
    Species: Human
    Sample Type: Serum
    Application: WB
  2. Hyaluronic acid and thrombin upregulate MT1-MMP through PI3K and Rac-1 signaling and prime the homing-related responses of cord blood hematopoietic stem/progenitor cells.
    Authors: Shirvaikar N, Marquez-Curtis LA, Ratajczak MZ, Janowska-Wieczorek A
    Stem Cells Dev., 2011;20(0):19-30.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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