Human MMP-14/MT1-MMP Antibody Summary
-8, -9, -10, -12, -13 or the catalytic domains of rhMMP-15 and -16.
Accession # P50281
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human MMP‑14/MT1‑MMP by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP‑14 in Human Benign Nodular Hyperplasia. MMP-14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP-14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and Integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (2).
Citations for Human MMP-14/MT1-MMP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Selective blockade of matrix metalloprotease-14 with a monoclonal antibody abrogates invasion, angiogenesis, and tumor growth in ovarian cancer.
Authors: Kaimal R, Aljumaily R, Tressel S, Pradhan R, Covic L, Kuliopulos A, Zarwan C, Kim Y, Sharifi S, Agarwal A
Cancer Res, 2013;73(8):2457-67.
Sample Types: Serum
Applications: Western Blot
Hyaluronic acid and thrombin upregulate MT1-MMP through PI3K and Rac-1 signaling and prime the homing-related responses of cord blood hematopoietic stem/progenitor cells.
Authors: Shirvaikar N, Marquez-Curtis LA, Ratajczak MZ, Janowska-Wieczorek A
Stem Cells Dev., 2011;20(0):19-30.
Sample Types: Whole Cells
Laminin-111 derived peptides AG73 and C16 regulate invadopodia activity of a human adenoid cystic carcinoma cell line.
Authors: Nascimento C, de Siqueira A, Pinheiro J, Freitas V, Jaeger R
Exp Cell Res, 0;317(18):2562-72.
Sample Types: Whole Cells
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