Human MMP-15/MT2-MMP Antibody

Catalog # Availability Size / Price Qty
MAB9161
MAB9161-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human MMP-15/MT2-MMP Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP‑15/MT2‑MMP in direct ELISAs and Western blots. Does not detect the catalytic domain of E. coli-expressed recombinant human MMP-15 (aa 132‑304).
Source
Monoclonal Mouse IgG1 Clone # 130522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-15
aa 132-625
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL

Recombinant Human MMP-15 Catalytic Domain

Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human renal cancer tissue
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
SW480 human colorectal adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-15/MT2-MMP

MMP-15, also known as membrane-type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.

Long Name
Matrix Metalloproteinase 15/Membrane Type 2 MMP
Entrez Gene IDs
4324 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane-inserted); Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase; MMP15; MMP-15; MT2-MMP; MT2-MMPMT2MMP; MTMMP2MT-MMP 2; SMCP-2matrix metalloproteinase-15

Product Datasheets

Citation for Human MMP-15/MT2-MMP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MMP-15 Is Upregulated in Preeclampsia, but Does Not Cleave Endoglin to Produce Soluble Endoglin.
    Authors: Kaitu'u-Lino TJ, Palmer K, Tuohey L, Ye L, Tong S
    PLoS ONE, 2012;7(6):e39864.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-15/MT2-MMP Antibody

Average Rating: 4 (Based on 1 Review)

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Human MMP-15/MT2-MMP Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human