Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 130522
View all MMP-15/MT2-MMP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-15
aa 132-625

Specificity

Detects human MMP‑15/MT2‑MMP in direct ELISAs and Western blots. Does not detect the catalytic domain of E. coli-expressed recombinant human MMP-15 (aa 132‑304).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human MMP‑15/MT2‑MMP Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human renal cancer tissue

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: SW480 human colorectal adenocarcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample:

Recombinant Human MMP-15 Catalytic Domain

Reviewed Applications

Read 2 reviews rated 3.5 using MAB9161 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MMP-15/MT2-MMP

MMP-15, also known as membrane-type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.

Long Name

Matrix Metalloproteinase 15/Membrane Type 2 MMP

Alternate Names

MMP15, MT2-MMP

Entrez Gene IDs

4324 (Human)

Gene Symbol

MMP15

Additional MMP-15/MT2-MMP Products

Product Documents for Human MMP‑15/MT2‑MMP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑15/MT2‑MMP Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP‑15/MT2‑MMP Antibody

Customer Reviews for Human MMP‑15/MT2‑MMP Antibody (2)

3.5 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
50%
3 Stars
50%
2 Stars
0%
1 Stars
0%

Have you used Human MMP‑15/MT2‑MMP Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human MMP-15/MT2-MMP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human MMP‑15/MT2‑MMP Antibody MAB9161
  • Human MMP-15/MT2-MMP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies