Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 130516
View all MMP-15/MT2-MMP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-15
aa 132-625

Specificity

Detects human MMP‑15/MT2‑MMP Catalytic Domain in Western blots. In Western blots, no cross-reactivity with the catalytic domain of recombinant human MMP-14 (MT1-MMP) or rhMMP-16 (MT3-MMP) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human MMP‑15/MT2‑MMP Catalytic Domain Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑15, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑15 Catalytic Domain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-15/MT2-MMP

MMP-15, also known as membrane-type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.

Long Name

Matrix Metalloproteinase 15/Membrane Type 2 MMP

Alternate Names

MMP15, MT2-MMP

Entrez Gene IDs

4324 (Human)

Gene Symbol

MMP15

Additional MMP-15/MT2-MMP Products

Product Documents for Human MMP‑15/MT2‑MMP Catalytic Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑15/MT2‑MMP Catalytic Domain Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP‑15/MT2‑MMP Catalytic Domain Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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