Human MMP-16/MT3-MMP Antibody Summary
Ala32-Gly291 (Ile152Asn)
Accession # P51512
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
MMP‑16/MT3‑MMP in Human Breast. MMP-16/MT3-MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP-16/MT3-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
Detection of MMP-16/MT3-MMP by Western Blot Mechanism: effect of miR-193b-3p’s target gene MMP16 on chondrocyte sheets. (A) Sequence of the predicted miR-193b-3p binding site with the 3’UTR of MMP16 (position 2232-2238). (B) Vectors used in dual-luciferase reporter assay containing the seed sequence of MMP16 3’UTR. (C) Reduced relative luciferase activity transfected with MMP16 and miR-193b-3p, compared with the negative control or MMP16 mutant (p < 0.05) (n=3). (D, E) Gene expression of COL2A1 and MMP16 (p < 0.05) (n=3). (F) Protein expression of COL2A1 and MMP16 for chondrocytes cultured in different groups. (G, H) Quantitative protein level of COL2A1 and MMP16 in different groups (p < 0.05) (n=3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31492826), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-16/MT3-MMP
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5). The rhMMP-16PC consists of the pro and catalytic domains, which can be activated by treatment with furin.
- Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
- Shofuda, K. et al. (1997) J. Biol. Chem. 272:9749.
- Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
- Kang, T. et al. (2000) FASEB J. 14:2559.
- Lang, R. et al. (2004) J. Mol. Biol. 336:213.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human MMP-16/MT3-MMP Antibody
There are currently no reviews for this product. Be the first to review Human MMP-16/MT3-MMP Antibody and earn rewards!
Have you used Human MMP-16/MT3-MMP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
