Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, ELISA (detection), ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 462732
View all BMP-10 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn317-Arg424
Accession # O95393

Specificity

Detects human BMP-10 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Mouse BMP‑10 Antibody

Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody.

Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody.

Recombinant Human BMP-10 (Catalog # 2926-BP) induces alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-10 Monoclonal Antibody (Catalog # MAB2926). The ND50 is typically 0.15-0.9 µg/mL.

Applications for Human/Mouse BMP‑10 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human BMP‑10 (Catalog # 2926-BP)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize BMP‑10-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. The Neutralization Dose (ND50) is typically 0.15-0.9 µg/mL in the presence of 100 ng/mL Recombinant Human BMP‑10.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-10

Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 295 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. Mature human BMP-10 shares 98% aa sequence identity with mature mouse, and rat BMP-10.

Long Name

Bone Morphogenetic Protein 10

Alternate Names

BMP10

Entrez Gene IDs

27302 (Human); 12154 (Mouse)

Gene Symbol

BMP10

UniProt

O95393

Additional BMP-10 Products

Product Documents for Human/Mouse BMP‑10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse BMP‑10 Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human/Mouse BMP‑10 Antibody

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