Human/Mouse BMP-10 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human BMP-10 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BMP-1.1, 2, 3, 3b, 4, 5, 6, 7, 9, 15, or the pro region of rhBMP-10 is observed. In direct ELISAs, approximately 90% cross-reactivity with recombinant mouse BMP-10 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 462732
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
    Asn317-Arg424
    Accession # O95393
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human BMP‑10 (Catalog # 2926-BP)
    under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize BMP‑10-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 100 ng/mL Recombinant Human BMP‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Alkaline Phosphatase Production Induced by BMP‑10 and Neutralization by Human BMP‑10 Antibody. Recombinant Human BMP‑10 (Catalog # 2926-BP) induces alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑10 Monoclonal Antibody (Catalog # MAB2926). The ND50 is typically 0.1‑0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-10
Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 295 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. Mature human BMP-10 shares 98% aa sequence identity with mature mouse, and rat BMP-10.

  • Long Name:
    Bone Morphogenetic Protein 10
  • Entrez Gene IDs:
    27302 (Human)
  • Alternate Names:
    BMP10; BMP-10; bone morphogenetic protein 10; MGC126783
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. BMP9 and BMP10 are necessary for proper closure of the ductus arteriosus.
    Authors: Levet S, Ouarne M, Ciais D, Coutton C, Subileau M, Mallet C, Ricard N, Bidart M, Debillon T, Faravelli F, Rooryck C, Feige J, Tillet E, Bailly S
    Proc Natl Acad Sci U S A, 2015;112(25):E3207-15.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
    Blood, 2012;119(25):6162-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.
    Authors: Kahr PC, Piccini I, Fabritz L, Greber B, Scholer H, Scheld HH, Hoffmeier A, Brown NA, Kirchhof P
    PLoS ONE, 2011;6(10):e26389.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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