Human/Mouse/Bovine Insulin Antibody

Catalog # Availability Size / Price Qty
MAB1417
MAB1417-SP
Insulin in  beta TC‑6 Mouse Cell Line.
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Product Details
Citations (6)
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Human/Mouse/Bovine Insulin Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects bovine, human, and mouse insulin.
Source
Monoclonal Rat IgG2A Clone # 182410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Insulin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
beta TC‑6 mouse beta cell insulinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Insulin in  beta TC‑6 Mouse Cell Line. View Larger

Insulin in beta TC‑6 Mouse Cell Line. Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

Product Datasheets

Citations for Human/Mouse/Bovine Insulin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Macrophage contact dependent and independent TLR4 mechanisms induce beta-cell dysfunction and apoptosis in a mouse model of type 2 diabetes.
    Authors: Cucak H, Mayer C, Tonnesen M, Thomsen L, Grunnet L, Rosendahl A
    PLoS ONE, 2014;9(3):e90685.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization.
    Authors: Cucak H, Grunnet L, Rosendahl A
    J Leukoc Biol, 2014;95(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes.
    Authors: Hagberg C, Mehlem A, Falkevall A, Muhl L, Fam B, Ortsater H, Scotney P, Nyqvist D, Samen E, Lu L, Stone-Elander S, Proietto J, Andrikopoulos S, Sjoholm A, Nash A, Eriksson U
    Nature, 2012;490(7420):426-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  4. Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus.
    Authors: Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM
    PLoS ONE, 2011;6(12):e29166.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.
    Authors: Jin CX, Li WL, Xu F, Geng ZH, He ZY, Su J, Tao XR, Ding XY, Wang X, Hu YP
    J. Cell. Biochem., 2008;104(1):224-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress.
    Authors: Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M
    Proteomics, 2006;6(24):6578-87.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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