Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cyclin B2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Cyclin B2
Ala2-Leu101
Accession # P30276

Specificity

Detects human and mouse Cyclin B2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Cyclin B2 Antibody

Detection of Human and Mouse Cyclin B2 antibody by Western Blot.

Detection of Human and Mouse Cyclin B2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin B2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Cyclin B2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin B2

Cyclin B2 (also CCNB2 and G2/mitotic-specific Cyclin B2) is a 48-54 kDa member of the cyclin AB subfamily, cyclin family of proteins. It is widely expressed and associates with CDK1. Here, it provides substrate specificity to a phosphorylating complex. A phosphor‑CDK1:Cyclin B2 complex is associated with the Golgi apparatus, where it contributes to Golgi fragmentation during mitosis. It is also possible that Cyclin B2 can substitute for Cyclin B1 during the early stages of mitosis. Human Cyclin B2 is 398 amino acids (aa) in length. It contains two cyclin box folds (aa 201‑290 and 298‑383) and two substrate binding sites (aa 165‑254 and 264‑347). Phosphorylation occurs at Ser92, Thr94, Ser99, Ser204, Ser392 and Ser398. Over aa 1‑101, human Cyclin B2 shares 79% aa identity with mouse Cyclin B2.

Alternate Names

CCNB2

Entrez Gene IDs

9133 (Human); 12442 (Mouse); 363088 (Rat)

Gene Symbol

CCNB2

UniProt

P30276

Additional Cyclin B2 Products

Product Documents for Human/Mouse Cyclin B2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Cyclin B2 Antibody

For research use only

Citations for Human/Mouse Cyclin B2 Antibody

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