Human/Mouse DAPP1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse DAPP1 by Western Blot.
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Product Details
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Human/Mouse DAPP1 Antibody Summary

Species Reactivity
Human, Mouse
Detects human DAPP1 in direct ELISAs. Detects human and mouse DAPP1 in Western blots.
Monoclonal Mouse IgG2A Clone # 716804
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DAPP1
Accession # Q9UN19
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse DAPP1 antibody by Western Blot. View Larger

Detection of Human and Mouse DAPP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse DAPP1 Monoclonal Antibody (Catalog # MAB7024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAPP1

DAPP1, also known as Bam32, is a 32 kDa intracellular adaptor protein for tyrosine-phosphorylated proteins and 3-phosphoinositides. It is expressed in B cells, T cells, and mast cells and plays a role in signal transduction during the activation of these cell types. DAPP1 is also required for progression of germinal center reactions and the adhesion of B cells to T cells. It is recruited to the plasma membrane following PI 3-kinase activation. DAPP1 contains one SH2 (aa 34-129) and one PH (aa 164-259) domain. Within aa 2-163, human DAPP1 shares approximately 90% aa sequence identity with mouse and rat DAPP1. Alternate splicing generates an additional isoform with a 22 aa substitution at the C-terminus.

Long Name
Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1
Entrez Gene IDs
27071 (Human); 26377 (Mouse); 362046 (Rat)
Alternate Names
B lymphocyte adapter protein Bam32; BAM32; BAM32DKFZp667E0716; B-cell adapter molecule of 32 kDa; DAPP1; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; hDAPP1

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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