Human/Mouse FABP4/A-FABP Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse FABP4/A‑FABP by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human/Mouse FABP4/A-FABP Antibody Summary

Species Reactivity
Human, Mouse
Detects human FABP4/A‑FABP in direct ELISAs and human and mouse FABP4/A-FABP in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) FABP4, 50% cross-reactivity with recombinant human (rh) FABP3, 15% cross-reactivity with rhFABP3, and no cross-reactivity with rhFABP8 or rhFABP9 is observed.
Monoclonal Rat IgG1 Clone # 804914
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FABP4/A‑FABP
Accession # P15090
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for  a complete optimized ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse FABP4/A-FABP antibody by Western Blot. View Larger

Detection of Human and Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human/Mouse FABP4/A‑FABP ELISA Standard Curve. Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (Catalog # MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Long Name
Fatty Acid-Binding Protein 4
Entrez Gene IDs
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

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Citation for Human/Mouse FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes
    Authors: I Kladnicka, M Cedikova, J Jedlicka, M Kohoutova, L Muller, I Plavinova, M Kripnerova, M Bludovska, J Kuncova, D Mullerova
    Biomolecules, 2021;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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