Human FABP4/A-FABP Antibody

Human/Mouse FABP4/A‑FABP ELISA Standard Curve.

Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of FABP4 by Immunohistochemistry

Expression profiles of FABP4 and FABP5 in normal breast tissue. A–D Representative hematoxylin and eosin (H&E) staining of normal breast tissue, showing lobules (A), ducts (B), adipocytes (C), and blood vessels (D). Scale bar: 100 μm. E–H Representative immunostaining for FABP4 (red) in normal breast tissue, including lobules (E), ducts (F), adipocytes (G), and blood vessels (H). Scale bar: 100 μm. I, J Analysis of colocalization of vWF (brown) (I) and FABP4 (red) (J) expression in normal breast tissue. Scale bar: 100 μm. K, L Analysis of colocalization of CD163 (brown) (K) and FABP4 (red) (L) expression in normal breast tissue. Scale bar: 100 μm. M–O Representative immunostaining for FABP5 (red) and vWF (brown) in normal breast tissue, showing adipocytes (M), blood vessels (N), and epithelium (O). Scale bar: 100 μm. P Comparison of FABP4 and FABP5 expression levels across different cell types in normal breast tissue, including epithelium, myoepithelium, adipocytes, endothelium, and macrophages (n = 9 for each group). The scoring on a 1-to-5 scale was performed for all cells. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s12672-025-02117-x), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FABP4 by Immunohistochemistry

FABP4 expression pattern in breast cancer tissue. A–D Representative H&E staining of ductal carcinoma in situ (DCIS), including DCIS regions (A), surrounding immune cells (B), adipocytes (C), and stromal vessel (D). Scale bars: 100 μm. E–J Representative immunostaining for FABP4 (red), CD163 (brown) and VWF (brown) in DCIS breast tissue, including DCIS regions (E), immune cells (F), adipocytes (G), and stromal vessels (H). Scale bars: 100 μm. I–M Representative immunostaining for FABP4 in invasive frontline tumor cells and adjacent adipose tissue. Most tumor cells were negative for FABP4 (J). Tumor cells close to adipocytes were positive for FABP4 (black arrow) (K). Adipocytes adjacent to tumor cells were small (L). Adipocytes away from tumor were large (M). Scale bar for I: 200 μm, scale bar for panel J-M: 100 μm, (N) Comparison of adipocyte sizes at varying distances from tumor cells (p < 0.0001, n = 40 cells). Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s12672-025-02117-x), licensed under a CC-BY license. Not internally tested by R&D Systems.