Human FABP4/A-FABP Antibody
Human FABP4/A-FABP Antibody Summary
Cys2-Ala132
Accession # P15090
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Applications
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (Catalog # MAB3150).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat FABP4/A‑FABP by Western Blot. Western blot shows lysates of human heart tissue, rat heart tissue, mouse heart tissue, human adipose tissue, rat adipose tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FABP4/A‑FABP in Human Bladder Cancer Tissue. FABP4/A-FABP was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human and Mouse FABP4/A‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP4/A-FABP at approximately 19 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Human/Mouse FABP4/A‑FABP ELISA Standard Curve. Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP4/A-FABP
FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.
Product Datasheets
Citations for Human FABP4/A-FABP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration
Authors: C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu
Cell Res., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration
Authors: C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu
Cell Res., 2020;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites
Authors: C Bosc, N Broin, M Fanjul, E Saland, T Farge, C Courdy, A Batut, R Masoud, C Larrue, S Skuli, N Espagnolle, JC Pagès, A Carrier, F Bost, J Bertrand-M, J Tamburini, C Récher, S Bertoli, V Mansat-De, S Manenti, JE Sarry, C Joffre
Nat Commun, 2020;11(1):4056.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Patient Adipose Stem Cell-Derived Adipocytes Reveal Genetic Variation that Predicts Antidiabetic Drug Response
Authors: W Hu, C Jiang, D Guan, P Dierickx, R Zhang, A Moscati, GN Nadkarni, DJ Steger, RJF Loos, C Hu, W Jia, RE Soccio, MA Lazar
Cell Stem Cell, 2019;24(2):299-308.e6.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Firemaster� 550 and its components isopropylated triphenyl phosphate and triphenyl phosphate enhance adipogenesis and transcriptional activity of peroxisome proliferator activated receptor (Ppar?) on the adipocyte protein 2 (aP2) promoter
Authors: EWY Tung, S Ahmed, V Peshdary, E Atlas
PLoS ONE, 2017;12(4):e0175855.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of multipotent stem cells in human brain tissue following stroke
Authors: K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi
Stem Cells Dev, 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Transcriptome sequencing wide functional analysis of human mesenchymal stem cells in response to TLR4 ligand
Sci Rep, 2016;6(0):30311.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis.
Authors: Xia H, Bodempudi V, Benyumov A, Hergert P, Tank D, Herrera J, Braziunas J, Larsson O, Parker M, Rossi D, Smith K, Peterson M, Limper A, Jessurun J, Connett J, Ingbar D, Phan S, Bitterman P, Henke C
Am J Pathol, 2014;184(5):1369-83.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Authors: Reinhardt P, Glatza M, Hemmer K, Tsytsyura Y, Thiel C, Hoing S, Moritz S, Parga J, Wagner L, Bruder J, Wu G, Schmid B, Ropke A, Klingauf J, Schwamborn J, Gasser T, Scholer H, Sterneckert J
PLoS ONE, 2013;8(3):e59252.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
Authors: Klim JR, Li L, Wrighton PJ
Nat. Methods, 2010;7(12):989-94.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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