Human/Mouse FoxF1 Antibody

Catalog # Availability Size / Price Qty
AF4798
AF4798-SP
Detection  of Human FoxF1 by Western Blot.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse FoxF1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse FoxF1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxF1
Met154-Met379
Accession # Q12946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human FoxF1 by Western Blot. View Larger

Detection of Human FoxF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Mouse FoxF1 by Western Blot. View Larger

Detection of Mouse FoxF1 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxF1

FoxF1 belongs to a large family of proteins that share a common forkhead/winged helix DNA binding domain. FoxF1 is implicated in pulmonary morphogenesis, specifically in development of the lung mesenchyme. Experiments in mice indicate that haploinsufficiency of FoxF1 can lead to perinatal lethality due to pulmonary abnormalities. In addition expression of FoxF1 can be induced by hedgehog ligands and appears to regulate expression of BMP-4.

Long Name
Forkhead Box F1
Entrez Gene IDs
2294 (Human); 15227 (Mouse)
Alternate Names
FKHL5; FKHL5MGC105125; forkhead box F1; forkhead box protein F1; Forkhead, drosophila, homolog-like 5; Forkhead-related activator 1; Forkhead-related protein FKHL5; Forkhead-related transcription factor 1; FoxF1; FREAC-1; FREAC1ACDMPV

Product Datasheets

Citations for Human/Mouse FoxF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
    Authors: J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang
    Elife, 2019;8(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. FOXF1 Inhibits Pulmonary Fibrosis by Preventing CDH2-CDH11 Cadherin Switch in Myofibroblasts
    Authors: M Black, D Milewski, T Le, X Ren, Y Xu, VV Kalinichen, TV Kalin
    Cell Rep, 2018;23(2):442-458.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  3. Wnt/?-catenin promotes gastric fundus specification in mice and humans
    Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. FoxF1 and FoxF2 transcription factors synergistically promote rhabdomyosarcoma carcinogenesis by repressing transcription of p21(Cip1) CDK inhibitor
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. A Molecular atlas of Xenopus respiratory system development.
    Authors: Rankin S, Thi Tran H, Wlizla M, Mancini P, Shifley E, Bloor S, Han L, Vleminckx K, Wert S, Zorn A
    Dev Dyn, 2015;244(1):69-85.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse FoxF1 Antibody

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Human/Mouse FoxF1 Antibody
By Anonymous on 07/01/2018
Application: ICC/IF Sample Tested: human lung Species: Human