Detection of Human/Mouse FoxF1 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FoxF1 belongs to a large family of proteins that share a common forkhead/winged helix DNA binding domain. FoxF1 is implicated in pulmonary morphogenesis, specifically in development of the lung mesenchyme. Experiments in mice indicate that haploinsufficiency of FoxF1 can lead to perinatal lethality due to pulmonary abnormalities. In addition expression of FoxF1 can be induced by hedgehog ligands and appears to regulate expression of BMP-4.
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