Detection of Human HMGB3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 0.05 µg/mL Mouse Anti-Human/Mouse HMGB3 Monoclonal Antibody (Catalog # MAB5507) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HMGB3 was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
High mobility group protein B3 (HMGB3; also HMG-4 and HMG-2a) is a 23 kDa (predicted) nuclear protein and member of the HMGB family. Human HMGB3 is synthesized as a 200 amino acid precursor, which is demethylated to produce the 199 aa mature chain. The protein contains two HMG box DNA-binding domains (aa 9‑79 and 93‑161) and an acidic Asp/Glu-rich region (aa 181‑200). Human HMGB3 shares 98% aa sequence identity with mouse and bovine HMGB3. HMGB3 is expressed predominantly in the placenta. It binds preferentially single‑stranded DNA and unwinds double stranded DNA.
High Mobility Group Box 3
Entrez Gene IDs:
3149 (Human); 15354 (Mouse); 305373 (Rat)
High mobility group protein 2a; High mobility group protein 4; high mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; high-mobility group box 3; HMG2A; HMG-2a; HMG2AHMG-4; HMG4; HMG4MGC90319; non-histone chromosomal protein
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